2fco

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[[Image:2fco.gif|left|200px]]
[[Image:2fco.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2fco |SIZE=350|CAPTION= <scene name='initialview01'>2fco</scene>, resolution 1.40&Aring;
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The line below this paragraph, containing "STRUCTURE_2fco", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= RecU/PrfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 Geobacillus kaustophilus])
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|DOMAIN=
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{{STRUCTURE_2fco| PDB=2fco | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fco OCA], [http://www.ebi.ac.uk/pdbsum/2fco PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fco RCSB]</span>
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}}
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'''Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase'''
'''Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase'''
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[[Category: Jedrzejas, M J.]]
[[Category: Jedrzejas, M J.]]
[[Category: Li, J.]]
[[Category: Li, J.]]
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[[Category: crystal structure]]
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[[Category: Crystal structure]]
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[[Category: flexibility]]
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[[Category: Flexibility]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:44:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:00:58 2008''
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Revision as of 00:44, 4 May 2008

Image:2fco.gif

Template:STRUCTURE 2fco

Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase


Overview

Here we report a high resolution structure of RecU-Holliday junction resolvase from Bacillus stearothermophilus. The functional unit of RecU is a homodimer that contains a "mushroom" like structure with a rigid cap and two highly flexible loops extending outwards. These loops appear to be highly flexible/dynamic, and presumably are directly involved in DNA binding and holding it for catalysis. Structural modifications of both the protein and DNA upon their interaction are essential for catalysis. An Mg2+ ion is present in each of the two active sites in this homodimeric enzyme, and two water molecules are coordinated with each Mg2+ ion. Our data are consistent with one of these water molecules acting as a nucleophile and the other as a general acid. The identities of the general base and general acid involved in catalysis and the Lewis acid that stabilizes the pentacovalent transition state phosphate ion are proposed. A model for the RecU-Holliday junction DNA complex is also proposed and discussed in the context of DNA binding and cleavage.

About this Structure

2FCO is a Single protein structure of sequence from Geobacillus kaustophilus. Full crystallographic information is available from OCA.

Reference

Structure, flexibility, and mechanism of the Bacillus stearothermophilus RecU Holliday junction resolvase., Kelly SJ, Li J, Setlow P, Jedrzejas MJ, Proteins. 2007 Sep 1;68(4):961-71. PMID:17557334 Page seeded by OCA on Sun May 4 03:44:20 2008

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