2fe8
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2fe8.gif|left|200px]] | [[Image:2fe8.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2fe8", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_2fe8| PDB=2fe8 | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''SARS coronavirus papain-like protease: structure of a viral deubiquitinating enzyme''' | '''SARS coronavirus papain-like protease: structure of a viral deubiquitinating enzyme''' | ||
| Line 28: | Line 25: | ||
[[Category: Ratia, K.]] | [[Category: Ratia, K.]] | ||
[[Category: Santarsiero, B D.]] | [[Category: Santarsiero, B D.]] | ||
| - | [[Category: | + | [[Category: Protease]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:47:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:47, 4 May 2008
SARS coronavirus papain-like protease: structure of a viral deubiquitinating enzyme
Overview
Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. In this work, we describe the 1.85-A crystal structure of the catalytic core of SARS-CoV PLpro and show that the overall architecture adopts a fold closely resembling that of known deubiquitinating enzymes. Key features, however, distinguish PLpro from characterized deubiquitinating enzymes, including an intact zinc-binding motif, an unobstructed catalytically competent active site, and the presence of an intriguing, ubiquitin-like N-terminal domain. To gain insight into the active-site recognition of the C-terminal tail of ubiquitin and the related LXGG motif, we propose a model of PLpro in complex with ubiquitin-aldehyde that reveals well defined sites within the catalytic cleft that help to account for strict substrate-recognition motifs.
About this Structure
2FE8 is a Single protein structure of sequence from Sars coronavirus. Full crystallographic information is available from OCA.
Reference
Severe acute respiratory syndrome coronavirus papain-like protease: structure of a viral deubiquitinating enzyme., Ratia K, Saikatendu KS, Santarsiero BD, Barretto N, Baker SC, Stevens RC, Mesecar AD, Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5717-22. Epub 2006 Mar 31. PMID:16581910 Page seeded by OCA on Sun May 4 03:47:29 2008
