2fey - Proteopedia, life in 3D

Revision as of 00:48, 4 May 2008

The structure of stem loop IV of Tetrahymena telomerase RNA

Overview

Conserved domains within the RNA component of telomerase provide the template for reverse transcription, recruit protein components to the holoenzyme and are required for enzymatic activity. Among the functionally essential domains in ciliate telomerase RNA is stem-loop IV, which strongly stimulates telomerase activity and processivity even when provided in trans. The NMR structure of Tetrahymena thermophila stem-loop IV shows a highly structured distal stem-loop linked to a conformationally flexible template-proximal region by a bulge that severely kinks the entire RNA. Through extensive structure-function studies, we identify residues that contribute to both these structural features and to enzymatic activity, with no apparent effect on the binding of TERT protein. We propose that the bending induced by the GA bulge and the flexibility of the template-proximal region allow positioning of the prestructured apical loop during the catalytic cycle.

About this Structure

Full crystallographic information is available from OCA.

Reference

Structure of stem-loop IV of Tetrahymena telomerase RNA., Chen Y, Fender J, Legassie JD, Jarstfer MB, Bryan TM, Varani G, EMBO J. 2006 Jul 12;25(13):3156-66. Epub 2006 Jun 15. PMID:16778765 Page seeded by OCA on Sun May 4 03:48:58 2008

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 [[Image:2fey.gif|left|200px]]
- {{Structure
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-}}
 '''The structure of stem loop IV of Tetrahymena telomerase RNA'''
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 ==About this Structure==
-FEY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FEY OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FEY OCA].
 ==Reference==
 Structure of stem-loop IV of Tetrahymena telomerase RNA., Chen Y, Fender J, Legassie JD, Jarstfer MB, Bryan TM, Varani G, EMBO J. 2006 Jul 12;25(13):3156-66. Epub 2006 Jun 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16778765 16778765]
-[[Category: Protein complex]]
 [[Category: Bryan, T M.]]
 [[Category: Chen, Y.]]
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 [[Category: Legassie, J D.]]
 [[Category: Varani, G.]]
-[[Category: heptaloop]]
+[[Category: Heptaloop]]
-[[Category: nmr structure]]
+[[Category: Nmr structure]]
-[[Category: stem-loop iv]]
+[[Category: Stem-loop iv]]
-[[Category: telomerase rna]]
+[[Category: Telomerase rna]]
-[[Category: tetrahymena]]
+[[Category: Tetrahymena]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:48:58 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:01:55 2008''