This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.




2fid

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2fid.gif|left|200px]]
[[Image:2fid.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2fid |SIZE=350|CAPTION= <scene name='initialview01'>2fid</scene>, resolution 2.80&Aring;
+
The line below this paragraph, containing "STRUCTURE_2fid", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= RABGEF1, RABEX5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2fid| PDB=2fid | SCENE= }}
-
|RELATEDENTRY=[[2fif|2FIF]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fid OCA], [http://www.ebi.ac.uk/pdbsum/2fid PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fid RCSB]</span>
+
-
}}
+
'''Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin'''
'''Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin'''
Line 27: Line 24:
[[Category: Hurley, J H.]]
[[Category: Hurley, J H.]]
[[Category: Lee, S.]]
[[Category: Lee, S.]]
-
[[Category: helix]]
+
[[Category: Helix]]
-
[[Category: zinc finger]]
+
[[Category: Zinc finger]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:56:02 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:03:10 2008''
+

Revision as of 00:56, 4 May 2008

Image:2fid.gif

Template:STRUCTURE 2fid

Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin


Overview

Rabex-5 is an exchange factor for Rab5, a master regulator of endosomal trafficking. Rabex-5 binds monoubiquitin, undergoes covalent ubiquitination and contains an intrinsic ubiquitin ligase activity, all of which require an N-terminal A20 zinc finger followed immediately by a helix. The structure of the N-terminal portion of Rabex-5 bound to ubiquitin at 2.5-A resolution shows that Rabex-5-ubiquitin interactions occur at two sites. The first site is a new type of ubiquitin-binding domain, an inverted ubiquitin-interacting motif, which binds with approximately 29-microM affinity to the canonical Ile44 hydrophobic patch on ubiquitin. The second is a diaromatic patch on the A20 zinc finger, which binds with approximately 22-microM affinity to a polar region centered on Asp58 of ubiquitin. The A20 zinc-finger diaromatic patch mediates ubiquitin-ligase activity by directly recruiting a ubiquitin-loaded ubiquitin-conjugating enzyme.

About this Structure

2FID is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5., Lee S, Tsai YC, Mattera R, Smith WJ, Kostelansky MS, Weissman AM, Bonifacino JS, Hurley JH, Nat Struct Mol Biol. 2006 Mar;13(3):264-71. Epub 2006 Feb 5. PMID:16462746 Page seeded by OCA on Sun May 4 03:56:02 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2fid"
Personal tools