2fju

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[[Image:2fju.gif|left|200px]]
[[Image:2fju.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2fju |SIZE=350|CAPTION= <scene name='initialview01'>2fju</scene>, resolution 2.20&Aring;
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The line below this paragraph, containing "STRUCTURE_2fju", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GSP:5&#39;-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoinositide_phospholipase_C Phosphoinositide phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.11 3.1.4.11] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= RAC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), PLCB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2fju| PDB=2fju | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fju OCA], [http://www.ebi.ac.uk/pdbsum/2fju PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fju RCSB]</span>
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}}
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'''Activated Rac1 bound to its effector phospholipase C beta 2'''
'''Activated Rac1 bound to its effector phospholipase C beta 2'''
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[[Category: Snyder, J T.]]
[[Category: Snyder, J T.]]
[[Category: Sondek, J.]]
[[Category: Sondek, J.]]
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[[Category: protein-protein complex]]
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[[Category: Protein-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:58:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:03:44 2008''
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Revision as of 00:58, 4 May 2008

Image:2fju.gif

Template:STRUCTURE 2fju

Activated Rac1 bound to its effector phospholipase C beta 2


Contents

Overview

Although diverse signaling cascades require the coordinated regulation of heterotrimeric G proteins and small GTPases, these connections remain poorly understood. We present the crystal structure of the GTPase Rac1 bound to phospholipase C-beta2 (PLC-beta2), a classic effector of heterotrimeric G proteins. Rac1 engages the pleckstrin-homology (PH) domain of PLC-beta2 to optimize its orientation for substrate membranes. Gbetagamma also engages the PH domain to activate PLC-beta2, and these two activation events are compatible, leading to additive stimulation of phospholipase activity. In contrast to PLC-delta, the PH domain of PLC-beta2 cannot bind phosphoinositides, eliminating this mode of regulation. The structure of the Rac1-PLC-beta2 complex reveals determinants that dictate selectivity of PLC-beta isozymes for Rac GTPases over other Rho-family GTPases, and substitutions within PLC-beta2 abrogate its stimulation by Rac1 but not by Gbetagamma, allowing for functional dissection of this integral signaling node.

Disease

Known disease associated with this structure: Platelet PLC beta-2 deficiency OMIM:[604114]

About this Structure

2FJU is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of Rac1 bound to its effector phospholipase C-beta2., Jezyk MR, Snyder JT, Gershberg S, Worthylake DK, Harden TK, Sondek J, Nat Struct Mol Biol. 2006 Dec;13(12):1135-40. Epub 2006 Nov 19. PMID:17115053 Page seeded by OCA on Sun May 4 03:58:56 2008

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OCA

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