2flg
From Proteopedia
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[[Image:2flg.gif|left|200px]] | [[Image:2flg.gif|left|200px]] | ||
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'''Solution structure of an EGF-LIKE domain from the Plasmodium falciparum merozoite surface protein 1''' | '''Solution structure of an EGF-LIKE domain from the Plasmodium falciparum merozoite surface protein 1''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FLG is a [[Single protein]] structure | + | 2FLG is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pluschke, G.]] | [[Category: Pluschke, G.]] | ||
[[Category: Robinson, J.]] | [[Category: Robinson, J.]] | ||
| - | [[Category: | + | [[Category: Egf-like domain]] |
| - | [[Category: | + | [[Category: Extracellular]] |
| - | [[Category: | + | [[Category: Malaria vaccine component]] |
| - | [[Category: | + | [[Category: Modular protein]] |
| - | [[Category: | + | [[Category: Surface antigen]] |
| - | [[Category: | + | [[Category: Surface protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:01:51 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:01, 4 May 2008
Solution structure of an EGF-LIKE domain from the Plasmodium falciparum merozoite surface protein 1
Overview
The Plasmodium falciparum merozoite surface protein-1 19 kDa fragment (MSP-1(19)) comprises two closely packed EGF-like domains (EGF=epidermal growth factor), each stabilized by three disulfide bonds. The native conformation of this protein is important for eliciting P. falciparum growth inhibitory antibodies. Here we show that the N-terminal EGF domain alone can be chemically synthesized and efficiently refolded to a native-like state, as shown by its solution structure as determined by NMR spectroscopy. In order to study its immunogenicity, the domain was coupled through its N terminus to a phospholipid and incorporated into reconstituted influenza virus-like particles (virosomes). When used to immunize mice, the peptide-loaded virosomes elicited potent humoral immune responses that were shown by Western blots and immunofluorescence assays to cross-react with native MSP-1 on the surfaces of P. falciparum blood stage parasites. This opens the way for a medicinal chemistry-oriented approach to the study and optimization of the antigenicity of the protein as a potential malaria vaccine candidate, whilst exploiting the immunopotentiating properties of influenza virosomes as a delivery vehicle.
About this Structure
2FLG is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Synthesis, solution structure and immune recognition of an epidermal growth factor-like domain from Plasmodium falciparum merozoite surface protein-1., James S, Moehle K, Renard A, Mueller MS, Vogel D, Zurbriggen R, Pluschke G, Robinson JA, Chembiochem. 2006 Dec;7(12):1943-50. PMID:17068840 Page seeded by OCA on Sun May 4 04:01:51 2008
