2fok

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[[Image:2fok.jpg|left|200px]]
[[Image:2fok.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2fok |SIZE=350|CAPTION= <scene name='initialview01'>2fok</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_2fok", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= FOKI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=244 Planomicrobium okeanokoites])
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-->
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|DOMAIN=
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{{STRUCTURE_2fok| PDB=2fok | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fok OCA], [http://www.ebi.ac.uk/pdbsum/2fok PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fok RCSB]</span>
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}}
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'''STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI'''
'''STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI'''
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[[Category: Schildkraut, I.]]
[[Category: Schildkraut, I.]]
[[Category: Wah, D A.]]
[[Category: Wah, D A.]]
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[[Category: deoxyribonuclease]]
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[[Category: Deoxyribonuclease]]
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[[Category: dna cleavage]]
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[[Category: Dna cleavage]]
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[[Category: dna hydrolysis]]
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[[Category: Dna hydrolysis]]
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[[Category: dna-binding protein]]
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[[Category: Dna-binding protein]]
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[[Category: metal ion catalysis]]
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[[Category: Metal ion catalysis]]
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[[Category: metalloenzyme]]
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[[Category: Metalloenzyme]]
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[[Category: nucleic acid recognition]]
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[[Category: Nucleic acid recognition]]
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[[Category: type iis restriction endonuclease]]
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[[Category: Type iis restriction endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:08:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:05:37 2008''
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Revision as of 01:08, 4 May 2008

Image:2fok.jpg

Template:STRUCTURE 2fok

STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI


Overview

FokI is a member an unusual class of restriction enzymes that recognize a specific DNA sequence and cleave nonspecifically a short distance away from that sequence. FokI consists of an N-terminal DNA recognition domain and a C-terminal cleavage domain. The bipartite nature of FokI has led to the development of artificial enzymes with novel specificities. We have solved the structure of FokI to 2.3 A resolution. The structure reveals a dimer, in which the dimerization interface is mediated by the cleavage domain. Each monomer has an overall conformation similar to that found in the FokI-DNA complex, with the cleavage domain packing alongside the DNA recognition domain. In corroboration with the cleavage data presented in the accompanying paper in this issue of Proceedings, we propose a model for FokI DNA cleavage that requires the dimerization of FokI on DNA to cleave both DNA strands.

About this Structure

2FOK is a Single protein structure of sequence from Planomicrobium okeanokoites. Full crystallographic information is available from OCA.

Reference

Structure of FokI has implications for DNA cleavage., Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK, Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9. PMID:9724743 Page seeded by OCA on Sun May 4 04:08:20 2008

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