2fpq

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[[Image:2fpq.gif|left|200px]]
[[Image:2fpq.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2fpq |SIZE=350|CAPTION= <scene name='initialview01'>2fpq</scene>, resolution 1.65&Aring;
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The line below this paragraph, containing "STRUCTURE_2fpq", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= BOTD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])
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-->
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|DOMAIN=
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{{STRUCTURE_2fpq| PDB=2fpq | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpq OCA], [http://www.ebi.ac.uk/pdbsum/2fpq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fpq RCSB]</span>
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}}
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'''Crystal Structure of Botulinum Neurotoxin Type D Light Chain'''
'''Crystal Structure of Botulinum Neurotoxin Type D Light Chain'''
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[[Category: Christian, T.]]
[[Category: Christian, T.]]
[[Category: Stevens, R C.]]
[[Category: Stevens, R C.]]
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[[Category: hexxh metalloprotease]]
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[[Category: Hexxh metalloprotease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:10:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:06:05 2008''
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Revision as of 01:10, 4 May 2008

Image:2fpq.gif

Template:STRUCTURE 2fpq

Crystal Structure of Botulinum Neurotoxin Type D Light Chain


Overview

The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 amino acids in length depending on the serotype. However, the structural details involved in substrate recognition remain largely unknown. Here is reported the 1.65 A resolution crystal structure of the catalytic domain of BoNT serotype D (BoNT/D-LC), providing insight into the protein-protein binding interaction and final proteolysis of VAMP-2. Structural analysis has identified a hydrophobic pocket potentially involved in substrate recognition of the P1' VAMP residue (Leu 60) and a second remote site for recognition of the V1 SNARE motif that is critical for activity. A structural comparison of BoNT/D-LC with BoNT/F-LC that also recognizes VAMP-2 one residue away from the BoNT/D-LC site provides additional molecular details about the unique serotype specific activities. In particular, BoNT/D prefers a hydrophobic interaction for the V1 motif of VAMP-2, while BoNT/F adopts a more hydrophilic strategy for recognition of the same V1 motif.

About this Structure

2FPQ is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.

Reference

Structure of botulinum neurotoxin type D light chain at 1.65 A resolution: repercussions for VAMP-2 substrate specificity., Arndt JW, Chai Q, Christian T, Stevens RC, Biochemistry. 2006 Mar 14;45(10):3255-62. PMID:16519520 Page seeded by OCA on Sun May 4 04:10:31 2008

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