2fq1

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[[Image:2fq1.gif|left|200px]]
[[Image:2fq1.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2fq1 |SIZE=350|CAPTION= <scene name='initialview01'>2fq1</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_2fq1", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismatase Isochorismatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.1 3.3.2.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= entB, entG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_2fq1| PDB=2fq1 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fq1 OCA], [http://www.ebi.ac.uk/pdbsum/2fq1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fq1 RCSB]</span>
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}}
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'''Crystal structure of the two-domain non-ribosomal peptide synthetase EntB containing isochorismate lyase and aryl-carrier protein domains'''
'''Crystal structure of the two-domain non-ribosomal peptide synthetase EntB containing isochorismate lyase and aryl-carrier protein domains'''
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[[Category: Gulick, A M.]]
[[Category: Gulick, A M.]]
[[Category: Nicolai, D A.]]
[[Category: Nicolai, D A.]]
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[[Category: acp]]
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[[Category: Acp]]
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[[Category: entb]]
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[[Category: Entb]]
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[[Category: multi-domain]]
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[[Category: Multi-domain]]
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[[Category: nrp]]
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[[Category: Nrp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:11:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:06:12 2008''
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Revision as of 01:11, 4 May 2008

Image:2fq1.gif

Template:STRUCTURE 2fq1

Crystal structure of the two-domain non-ribosomal peptide synthetase EntB containing isochorismate lyase and aryl-carrier protein domains


Overview

Nonribosomal peptide synthetases are modular proteins that operate in an assembly line fashion to bind, modify, and link amino acids. In the E. coli enterobactin NRPS system, the EntE adenylation domain catalyzes the transfer of a molecule of 2,3-dihydroxybenzoic acid to the pantetheine cofactor of EntB. We present here the crystal structure of the EntB protein that contains an N-terminal isochorismate lyase domain that functions in the synthesis of 2,3-dihydroxybenzoate and a C-terminal carrier protein domain. Functional analysis showed that the EntB-EntE interaction was surprisingly tolerant of a number of point mutations on the surface of EntB and EntE. Mutational studies on EntE support our previous hypothesis that members of the adenylate-forming family of enzymes adopt two distinct conformations to catalyze the two-step reactions.

About this Structure

2FQ1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain., Drake EJ, Nicolai DA, Gulick AM, Chem Biol. 2006 Apr;13(4):409-19. PMID:16632253 Page seeded by OCA on Sun May 4 04:11:24 2008

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