2nmo

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Revision as of 20:53, 12 November 2007

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spinqualitylabelsall models 2nmo, resolution 1.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of human galectin-3 carbohydrate-recognition domain at 1.35 angstrom resolution

Overview

Galectin-3 is a multifunctional carbohydrate-binding protein that has, roles in cancer progression. In addition to carbohydrate-dependent, extracellular functions, galectin-3 participates in, carbohydrate-independent intracellular signalling pathways, including, apoptosis, via protein-protein interactions, some of which engage the, carbohydrate-binding groove. When ligands bind within this site, conformational rearrangements are induced and information on unliganded, galectin-3 is therefore valuable for structure-based drug design. Removal, of cocrystallized lactose from the human galectin-3, carbohydrate-recognition domain was achieved via crystal soaking, but took, weeks despite low affinity. Pre-soaking to remove lactose enabled the, subsequent binding of cryoprotectant glycerol, whereas when the lactose, was not removed a priori the glycerol could not displace it in the short, cryosoaking time frame. This slow diffusion of lactose out of the crystals, contrasts with the entrance of glycerol, which takes place within minutes., The importance of the removal of incumbent ligands prior to attempts to, introduce alternative ligands is indicated, even for proteins exhibiting, low affinity for ligands, and has significance for ligand exchange in, structure-based drug design.

About this Structure

2NMO is a Single protein structure of sequence from Homo sapiens with CL and GOL as ligands. Full crystallographic information is available from OCA.

Reference

Slow diffusion of lactose out of galectin-3 crystals monitored by X-ray crystallography: possible implications for ligand-exchange protocols., Collins PM, Hidari KI, Blanchard H, Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):415-9. Epub 2007, Feb 21. PMID:17327679

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