This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2g1g
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2g1g.gif|left|200px]] | [[Image:2g1g.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2g1g", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_2g1g| PDB=2g1g | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine''' | '''Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G1G OCA]. | |
==Reference== | ==Reference== | ||
The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif., Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P, Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16738127 16738127] | The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif., Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P, Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16738127 16738127] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Lescrinier, E.]] | [[Category: Lescrinier, E.]] | ||
| - | [[Category: | + | [[Category: T-rnai anticodonloop t6a]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:35:18 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:35, 4 May 2008
Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine
Overview
Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem-loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet (t6A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t6A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A comparison of this stem-loop with our structure demonstrates different effects of the modification depending on the loop sequence.
About this Structure
Full crystallographic information is available from OCA.
Reference
The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif., Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P, Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:16738127 Page seeded by OCA on Sun May 4 04:35:18 2008
