2g1p
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2g1p.gif|left|200px]] | [[Image:2g1p.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2g1p", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_2g1p| PDB=2g1p | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structure of E. coli DNA adenine methyltransferase (DAM)''' | '''Structure of E. coli DNA adenine methyltransferase (DAM)''' | ||
| Line 25: | Line 22: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Site-specific DNA-methyltransferase (adenine-specific)]] | ||
[[Category: Bekes, M.]] | [[Category: Bekes, M.]] | ||
[[Category: Cheng, X.]] | [[Category: Cheng, X.]] | ||
| Line 31: | Line 27: | ||
[[Category: Jeltsch, A.]] | [[Category: Jeltsch, A.]] | ||
[[Category: Liebert, K.]] | [[Category: Liebert, K.]] | ||
| - | [[Category: | + | [[Category: Bacterial virulence factor]] |
| - | [[Category: | + | [[Category: Base flipping]] |
| - | [[Category: | + | [[Category: Dam methylation]] |
| - | [[Category: | + | [[Category: Gatc recognition]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:35:54 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:35, 4 May 2008
Structure of E. coli DNA adenine methyltransferase (DAM)
Overview
The structure of the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase in complex with cognate DNA was determined at 1.89 A resolution in the presence of S-adenosyl-L-homocysteine. DNA recognition and the dynamics of base-flipping were studied by site-directed mutagenesis, DNA methylation kinetics and fluorescence stopped-flow experiments. Our data illustrate the mechanism of coupling of DNA recognition and base-flipping. Contacts to the non-target strand in the second (3') half of the GATC site are established by R124 to the fourth base-pair, and by L122 and P134 to the third base-pair. The aromatic ring of Y119 intercalates into the DNA between the second and third base-pairs, which is essential for base-flipping to occur. Compared to previous published structures of bacteriophage T4 Dam, three major new observations are made in E.coli Dam. (1) The first Gua is recognized by K9, removal of which abrogates the first base-pair recognition. (2) The flipped target Ade binds to the surface of EcoDam in the absence of S-adenosyl-L-methionine, which illustrates a possible intermediate in the base-flipping pathway. (3) The orphaned Thy residue displays structural flexibility by adopting an extrahelical or intrahelical position where it is in contact to N120.
About this Structure
2G1P is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure and substrate recognition of the Escherichia coli DNA adenine methyltransferase., Horton JR, Liebert K, Bekes M, Jeltsch A, Cheng X, J Mol Biol. 2006 Apr 28;358(2):559-70. Epub 2006 Feb 28. PMID:16524590 Page seeded by OCA on Sun May 4 04:35:54 2008
