2g5h

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[[Image:2g5h.gif|left|200px]]
[[Image:2g5h.gif|left|200px]]
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{{Structure
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|PDB= 2g5h |SIZE=350|CAPTION= <scene name='initialview01'>2g5h</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_2g5h", creates the "Structure Box" on the page.
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|GENE= gatA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]), gatB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]), gatC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
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|DOMAIN=
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{{STRUCTURE_2g5h| PDB=2g5h | SCENE= }}
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|RELATEDENTRY=[[2f2a|2F2A]], [[2df4|2DF4]], [[2g5i|2G5I]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5h OCA], [http://www.ebi.ac.uk/pdbsum/2g5h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g5h RCSB]</span>
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'''Structure of tRNA-Dependent Amidotransferase GatCAB'''
'''Structure of tRNA-Dependent Amidotransferase GatCAB'''
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[[Category: Tanaka, I.]]
[[Category: Tanaka, I.]]
[[Category: Yao, M.]]
[[Category: Yao, M.]]
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[[Category: multi protein complex]]
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[[Category: Multi protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:42:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:12:03 2008''
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Revision as of 01:42, 4 May 2008

Image:2g5h.gif

Template:STRUCTURE 2g5h

Structure of tRNA-Dependent Amidotransferase GatCAB


Overview

The formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 A in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gln-tRNA(Gln).

About this Structure

2G5H is a Protein complex structure of sequences from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

Ammonia channel couples glutaminase with transamidase reactions in GatCAB., Nakamura A, Yao M, Chimnaronk S, Sakai N, Tanaka I, Science. 2006 Jun 30;312(5782):1954-8. PMID:16809541 Page seeded by OCA on Sun May 4 04:42:32 2008

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