2ga4

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[[Image:2ga4.gif|left|200px]]
[[Image:2ga4.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ga4 |SIZE=350|CAPTION= <scene name='initialview01'>2ga4</scene>, resolution 1.800&Aring;
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The line below this paragraph, containing "STRUCTURE_2ga4", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=1PS:3-PYRIDINIUM-1-YLPROPANE-1-SULFONATE'>1PS</scene>, <scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2ga4| PDB=2ga4 | SCENE= }}
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|RELATEDENTRY=[[1r4p|1R4P]], [[1r4q|1R4Q]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ga4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ga4 OCA], [http://www.ebi.ac.uk/pdbsum/2ga4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ga4 RCSB]</span>
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}}
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'''Stx2 with adenine'''
'''Stx2 with adenine'''
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[[Category: Enterobacteria phage 933w]]
[[Category: Enterobacteria phage 933w]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: rRNA N-glycosylase]]
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[[Category: RRNA N-glycosylase]]
[[Category: Fraser, M E.]]
[[Category: Fraser, M E.]]
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[[Category: ab5-toxin]]
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[[Category: Ab5-toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:52:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:13:51 2008''
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Revision as of 01:52, 4 May 2008

Image:2ga4.gif

Template:STRUCTURE 2ga4

Stx2 with adenine


Overview

Stx2 is a protein toxin whose catalytic subunit acts as an N-glycosidase to depurinate a specific adenine base from 28S rRNA. In the holotoxin, the catalytic portion, A1, is linked to the rest of the A subunit, A2, and A2 interacts with the pentameric ring formed by the five B subunits. In order to test whether the holotoxin is active as an N-glycosidase, Stx2 was crystallized in the presence of adenosine and adenine. The crystals diffracted to approximately 1.8 angstroms and showed clear electron density for adenine in the active site. Adenosine had been cleaved, proving that Stx2 is an active N-glycosidase. While the holotoxin is active against small substrates, it would be expected that the B subunits would interfere with the binding of the 28S rRNA.

About this Structure

2GA4 is a Protein complex structure of sequences from Enterobacteria phage 933w. Full crystallographic information is available from OCA.

Reference

Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7., Fraser ME, Cherney MM, Marcato P, Mulvey GL, Armstrong GD, James MN, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt, 7):627-30. Epub 2006 Jun 26. PMID:16820678 Page seeded by OCA on Sun May 4 04:52:30 2008

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