2gaj

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[[Image:2gaj.gif|left|200px]]
[[Image:2gaj.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2gaj |SIZE=350|CAPTION= <scene name='initialview01'>2gaj</scene>, resolution 1.950&Aring;
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The line below this paragraph, containing "STRUCTURE_2gaj", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= topA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
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|DOMAIN=
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{{STRUCTURE_2gaj| PDB=2gaj | SCENE= }}
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|RELATEDENTRY=[[1ecl|1ECL]], [[1d6m|1D6M]], [[1gku|1GKU]], [[2gai|2GAI]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gaj OCA], [http://www.ebi.ac.uk/pdbsum/2gaj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gaj RCSB]</span>
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}}
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'''Structure of Full Length Topoisomerase I from Thermotoga maritima in monoclinic crystal form'''
'''Structure of Full Length Topoisomerase I from Thermotoga maritima in monoclinic crystal form'''
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[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: Hansen, G.]]
[[Category: Hansen, G.]]
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[[Category: topoisomerase]]
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[[Category: Topoisomerase]]
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[[Category: zinc ribbon]]
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[[Category: Zinc ribbon]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:53:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:14:04 2008''
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Revision as of 01:53, 4 May 2008

Image:2gaj.gif

Template:STRUCTURE 2gaj

Structure of Full Length Topoisomerase I from Thermotoga maritima in monoclinic crystal form


Overview

DNA topoisomerases are a family of enzymes altering the topology of DNA by concerted breakage and rejoining of the phosphodiester backbone of DNA. Bacterial and archeal type IA topoisomerases, including topoisomerase I, topoisomerase III, and reverse gyrase, are crucial in regulation of DNA supercoiling and maintenance of genetic stability. The crystal structure of full length topoisomerase I from Thermotoga maritima was determined at 1.7A resolution and represents an intact and fully active bacterial topoisomerase I. It reveals the torus-like structure of the conserved transesterification core domain comprising domains I-IV and a tightly associated C-terminal zinc ribbon domain (domain V) packing against domain IV of the core domain. The previously established zinc-independence of the functional activity of T.maritima topoisomerase I is further supported by its crystal structure as no zinc ion is bound to domain V. However, the structural integrity is preserved by the formation of two disulfide bridges between the four Zn-binding cysteine residues. A functional role of domain V in DNA binding and recognition is suggested and discussed in the light of the structure and previous biochemical findings. In addition, implications for bacterial topoisomerases I are provided.

About this Structure

2GAJ is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

Crystal structure of full length topoisomerase I from Thermotoga maritima., Hansen G, Harrenga A, Wieland B, Schomburg D, Reinemer P, J Mol Biol. 2006 May 19;358(5):1328-40. Epub 2006 Mar 23. PMID:16600296 Page seeded by OCA on Sun May 4 04:53:17 2008

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