2ghj
From Proteopedia
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'''Crystal structure of folded and partially unfolded forms of Aquifex aeolicus ribosomal protein L20''' | '''Crystal structure of folded and partially unfolded forms of Aquifex aeolicus ribosomal protein L20''' | ||
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[[Category: Springer, M.]] | [[Category: Springer, M.]] | ||
[[Category: Timsit, Y.]] | [[Category: Timsit, Y.]] | ||
| - | [[Category: | + | [[Category: Folding intermediate]] |
| - | [[Category: | + | [[Category: Ribosomal protein extension]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:06:54 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:06, 4 May 2008
Crystal structure of folded and partially unfolded forms of Aquifex aeolicus ribosomal protein L20
Overview
The recent finding of intrinsically unstructured proteins defies the classical structure-function paradigm. However, owing to their flexibility, intrinsically unstructured proteins generally escape detailed structural investigations. Consequently little is known about the extent of conformational disorder and its role in biological functions. Here, we present the X-ray structure of the unbound ribosomal protein L20, the long basic amino-terminal extension of which has been previously interpreted as fully disordered in the absence of RNA. This study provides the first detailed picture of two protein folding states trapped together in a crystal and indicates that unfolding occurs in discrete regions of the whole protein, corresponding mainly to RNA-binding residues. The electrostatic destabilization of the long alpha-helix and a structural communication between the two L20 domains are reminiscent of those observed in calmodulin. The detailed comparison of the two conformations observed in the crystal provides new insights into the role of unfolded extensions in ribosomal assembly.
About this Structure
2GHJ is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Coexistence of two protein folding states in the crystal structure of ribosomal protein L20., Timsit Y, Allemand F, Chiaruttini C, Springer M, EMBO Rep. 2006 Oct;7(10):1013-8. Epub 2006 Sep 15. PMID:16977336 Page seeded by OCA on Sun May 4 05:06:54 2008
