2ght

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[[Image:2ght.gif|left|200px]]
[[Image:2ght.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ght |SIZE=350|CAPTION= <scene name='initialview01'>2ght</scene>, resolution 1.8&Aring;
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The line below this paragraph, containing "STRUCTURE_2ght", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= CTDSP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2ght| PDB=2ght | SCENE= }}
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|RELATEDENTRY=[[2ghq|2GHQ]], [[1ta0|1ta0]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ght FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ght OCA], [http://www.ebi.ac.uk/pdbsum/2ght PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ght RCSB]</span>
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}}
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'''CTD-specific phosphatase Scp1 in complex with peptide from C-terminal domain of RNA polymerase II'''
'''CTD-specific phosphatase Scp1 in complex with peptide from C-terminal domain of RNA polymerase II'''
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[[Category: Noel, J P.]]
[[Category: Noel, J P.]]
[[Category: Zhang, Y.]]
[[Category: Zhang, Y.]]
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[[Category: had superfamily]]
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[[Category: Had superfamily]]
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[[Category: protein-peptide complex]]
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[[Category: Protein-peptide complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:07:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:16:55 2008''
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Revision as of 02:07, 4 May 2008

Image:2ght.gif

Template:STRUCTURE 2ght

CTD-specific phosphatase Scp1 in complex with peptide from C-terminal domain of RNA polymerase II


Overview

Phosphorylation and dephosphorylation of the C-terminal domain (CTD) of RNA polymerase II (Pol II) represent a critical regulatory checkpoint for transcription. Transcription initiation requires Fcp1/Scp1-mediated dephosphorylation of phospho-CTD. Fcp1 and Scp1 belong to a family of Mg2+ -dependent phosphoserine (P.Ser)/phosphothreonine (P.Thr)-specific phosphatases. We recently showed that Scp1 is an evolutionarily conserved regulator of neuronal gene silencing. Here, we present the X-ray crystal structures of a dominant-negative form of human Scp1 (D96N mutant) bound to mono- and diphosphorylated peptides encompassing the CTD heptad repeat (Y1S2P3T4S5P6S7). Moreover, kinetic and thermodynamic analyses of Scp1-phospho-CTD peptide complexes support the structures determined. This combined structure-function analysis discloses the residues in Scp1 involved in CTD binding and its preferential dephosphorylation of P.Ser5 of the CTD heptad repeat. Moreover, these results provide a template for the design of specific inhibitors of Scp1 for the study of neuronal stem cell development.

About this Structure

2GHT is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1., Zhang Y, Kim Y, Genoud N, Gao J, Kelly JW, Pfaff SL, Gill GN, Dixon JE, Noel JP, Mol Cell. 2006 Dec 8;24(5):759-70. PMID:17157258 Page seeded by OCA on Sun May 4 05:07:30 2008

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