2gim

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[[Image:2gim.gif|left|200px]]
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{{Structure
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|PDB= 2gim |SIZE=350|CAPTION= <scene name='initialview01'>2gim</scene>, resolution 1.60&Aring;
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The line below this paragraph, containing "STRUCTURE_2gim", creates the "Structure Box" on the page.
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|GENE= petE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1172 Anabaena variabilis])
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{{STRUCTURE_2gim| PDB=2gim | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gim OCA], [http://www.ebi.ac.uk/pdbsum/2gim PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gim RCSB]</span>
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'''1.6 Angstrom structure of plastocyanin from Anabaena variabilis'''
'''1.6 Angstrom structure of plastocyanin from Anabaena variabilis'''
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[[Category: Harris, P.]]
[[Category: Harris, P.]]
[[Category: Schmidt, L.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:08:59 2008''
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Revision as of 02:09, 4 May 2008

Image:2gim.gif

Template:STRUCTURE 2gim

1.6 Angstrom structure of plastocyanin from Anabaena variabilis


Overview

Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.

About this Structure

2GIM is a Single protein structure of sequence from Anabaena variabilis. Full crystallographic information is available from OCA.

Reference

Structure of plastocyanin from the cyanobacterium Anabaena variabilis., Schmidt L, Christensen HE, Harris P, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:16929103 Page seeded by OCA on Sun May 4 05:08:59 2008

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