2gmr

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2gmr.gif|left|200px]]
[[Image:2gmr.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2gmr |SIZE=350|CAPTION= <scene name='initialview01'>2gmr</scene>, resolution 2.50&Aring;
+
The line below this paragraph, containing "STRUCTURE_2gmr", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= pufL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), pufM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), puhA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2gmr| PDB=2gmr | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gmr OCA], [http://www.ebi.ac.uk/pdbsum/2gmr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gmr RCSB]</span>
+
-
}}
+
'''Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn'''
'''Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn'''
Line 29: Line 26:
[[Category: Hofmann, E.]]
[[Category: Hofmann, E.]]
[[Category: Stachnik, J M.]]
[[Category: Stachnik, J M.]]
-
[[Category: electron transport]]
+
[[Category: Electron transport]]
-
[[Category: integral membrane protein]]
+
[[Category: Integral membrane protein]]
-
[[Category: l210dn]]
+
[[Category: L210dn]]
-
[[Category: mutant]]
+
[[Category: Mutant]]
-
[[Category: photosynthetic reaction center]]
+
[[Category: Photosynthetic reaction center]]
-
[[Category: proton transfer]]
+
[[Category: Proton transfer]]
-
[[Category: x-ray crystallography]]
+
[[Category: X-ray crystallography]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:17:00 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:18:44 2008''
+

Revision as of 02:17, 4 May 2008

Template:STRUCTURE 2gmr

Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn


Overview

The reaction center (RC) of Rhodobacter sphaeroides uses light energy to reduce and protonate a quinone molecule, QB (the secondary quinone electron acceptor), to form quinol, QBH2. Asp210 in the L-subunit has been shown to be a catalytic residue in this process. Mutation of Asp210 to Asn leads to a deceleration of reoxidation of QA- in the QA-QB --> QAQB- transition. Here we determined the structure of the Asp210 to Asn mutant to 2.5 A and show that there are no major structural differences as compared to the wild-type protein. We found QB in the distal position and a chain of water molecules between Asn210 and QB. Using time-resolved Fourier transform infrared (trFTIR) spectroscopy, we characterized the molecular reaction mechanism of this mutant. We found that QB- formation precedes QA- oxidation even more pronounced than in the wild-type reaction center. Continuum absorbance changes indicate deprotonation of a protonated water cluster, most likely of the water chain between Asn210 and QB. A detailed analysis of wild-type structures revealed a highly conserved water chain between Asp210 or Glu210 and QB in Rb. sphaeroides and Rhodopseudomonas viridis, respectively.

About this Structure

2GMR is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

Reference

Proton uptake in the reaction center mutant L210DN from Rhodobacter sphaeroides via protonated water molecules., Hermes S, Stachnik JM, Onidas D, Remy A, Hofmann E, Gerwert K, Biochemistry. 2006 Nov 21;45(46):13741-9. PMID:17105193 Page seeded by OCA on Sun May 4 05:17:00 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools