2gs0
From Proteopedia
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'''NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and the activation domain of p53''' | '''NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and the activation domain of p53''' | ||
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[[Category: Nguyen, B D.]] | [[Category: Nguyen, B D.]] | ||
[[Category: Omichinski, J G.]] | [[Category: Omichinski, J G.]] | ||
| - | [[Category: | + | [[Category: Activation]] |
| - | [[Category: | + | [[Category: P53]] |
| - | [[Category: | + | [[Category: Ph domain]] |
| - | [[Category: | + | [[Category: Tfb1]] |
| - | [[Category: | + | [[Category: Tfiih]] |
| - | [[Category: | + | [[Category: Transcription]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:27:06 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:27, 4 May 2008
NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and the activation domain of p53
Contents |
Overview
The interaction between the amino-terminal transactivation domain (TAD) of p53 and TFIIH is directly correlated with the ability of p53 to activate both transcription initiation and elongation. We have identified a region within the p53 TAD that specifically interacts with the pleckstrin homology (PH) domain of the p62 and Tfb1 subunits of human and yeast TFIIH. We have solved the 3D structure of a complex between the p53 TAD and the PH domain of Tfb1 by NMR spectroscopy. Our structure reveals that p53 forms a nine residue amphipathic alpha helix (residues 47-55) upon binding to Tfb1. In addition, we demonstrate that diphosphorylation of p53 at Ser46 and Thr55 leads to a significant enhancement in p53 binding to p62 and Tfb1. These results indicate that a phosphorylation cascade involving Ser46 and Thr55 of p53 could play an important role in the regulation of select p53 target genes.
Disease
Known disease associated with this structure: Adrenal cortical carcinoma OMIM:[191170], Breast cancer OMIM:[191170], Colorectal cancer OMIM:[191170], Hepatocellular carcinoma OMIM:[191170], Histiocytoma OMIM:[191170], Li-Fraumeni syndrome OMIM:[191170], Multiple malignancy syndrome OMIM:[191170], Nasopharyngeal carcinoma OMIM:[191170], Osteosarcoma OMIM:[191170], Pancreatic cancer OMIM:[191170], Thyroid carcinoma OMIM:[191170]
About this Structure
2GS0 is a Protein complex structure of sequences from Homo sapiens and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the Tfb1/p53 complex: Insights into the interaction between the p62/Tfb1 subunit of TFIIH and the activation domain of p53., Di Lello P, Jenkins LM, Jones TN, Nguyen BD, Hara T, Yamaguchi H, Dikeakos JD, Appella E, Legault P, Omichinski JG, Mol Cell. 2006 Jun 23;22(6):731-40. PMID:16793543 Page seeded by OCA on Sun May 4 05:27:06 2008
