2gsj

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[[Image:2gsj.jpg|left|200px]]
[[Image:2gsj.jpg|left|200px]]
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{{Structure
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|PDB= 2gsj |SIZE=350|CAPTION= <scene name='initialview01'>2gsj</scene>, resolution 1.73&Aring;
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The line below this paragraph, containing "STRUCTURE_2gsj", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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{{STRUCTURE_2gsj| PDB=2gsj | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gsj OCA], [http://www.ebi.ac.uk/pdbsum/2gsj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gsj RCSB]</span>
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'''cDNA cloning and 1.75A crystal structure determination of PPL2, a novel chimerolectin from Parkia platycephala seeds exhibiting endochitinolytic activity'''
'''cDNA cloning and 1.75A crystal structure determination of PPL2, a novel chimerolectin from Parkia platycephala seeds exhibiting endochitinolytic activity'''
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==About this Structure==
==About this Structure==
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2GSJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Parkia_platycephala Parkia platycephala]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSJ OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSJ OCA].
==Reference==
==Reference==
cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosamine-binding hemagglutinin from Parkia platycephala seeds., Cavada BS, Moreno FB, da Rocha BA, de Azevedo WF Jr, Castellon RE, Goersch GV, Nagano CS, de Souza EP, Nascimento KS, Radis-Baptista G, Delatorre P, Leroy Y, Toyama MH, Pinto VP, Sampaio AH, Barettino D, Debray H, Calvete JJ, Sanz L, FEBS J. 2006 Sep;273(17):3962-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16934035 16934035]
cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosamine-binding hemagglutinin from Parkia platycephala seeds., Cavada BS, Moreno FB, da Rocha BA, de Azevedo WF Jr, Castellon RE, Goersch GV, Nagano CS, de Souza EP, Nascimento KS, Radis-Baptista G, Delatorre P, Leroy Y, Toyama MH, Pinto VP, Sampaio AH, Barettino D, Debray H, Calvete JJ, Sanz L, FEBS J. 2006 Sep;273(17):3962-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16934035 16934035]
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[[Category: Parkia platycephala]]
 
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[[Category: Protein complex]]
 
[[Category: Barettino, D.]]
[[Category: Barettino, D.]]
[[Category: Calvete, J J.]]
[[Category: Calvete, J J.]]
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[[Category: Souza, E P.de.]]
[[Category: Souza, E P.de.]]
[[Category: Toyama, M H.]]
[[Category: Toyama, M H.]]
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[[Category: chimerolectin]]
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[[Category: Chimerolectin]]
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[[Category: endochitinase]]
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[[Category: Endochitinase]]
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[[Category: equilibrium sedimentation]]
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[[Category: Equilibrium sedimentation]]
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[[Category: glycosyl hydrolase family 18]]
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[[Category: Glycosyl hydrolase family 18]]
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[[Category: mimosoideae]]
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[[Category: Mimosoideae]]
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[[Category: parkia platycephala]]
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[[Category: Parkia platycephala]]
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[[Category: x-ray crystal structure]]
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[[Category: X-ray crystal structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:28:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:20:59 2008''
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Revision as of 02:28, 4 May 2008

Image:2gsj.jpg

Template:STRUCTURE 2gsj

cDNA cloning and 1.75A crystal structure determination of PPL2, a novel chimerolectin from Parkia platycephala seeds exhibiting endochitinolytic activity


Overview

Parkia platycephala lectin 2 was purified from Parkia platycephala (Leguminosae, Mimosoideae) seeds by affinity chromatography and RP-HPLC. Equilibrium sedimentation and MS showed that Parkia platycephala lectin 2 is a nonglycosylated monomeric protein of molecular mass 29 407+/-15 Da, which contains six cysteine residues engaged in the formation of three intramolecular disulfide bonds. Parkia platycephala lectin 2 agglutinated rabbit erythrocytes, and this activity was specifically inhibited by N-acetylglucosamine. In addition, Parkia platycephala lectin 2 hydrolyzed beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin. The full-length amino acid sequence of Parkia platycephala lectin 2, determined by N-terminal sequencing and cDNA cloning, and its three-dimensional structure, established by X-ray crystallography at 1.75 A resolution, showed that Parkia platycephala lectin 2 is homologous to endochitinases of the glycosyl hydrolase family 18, which share the (betaalpha)8 barrel topology harboring the catalytic residues Asp125, Glu127, and Tyr182.

About this Structure

Full crystallographic information is available from OCA.

Reference

cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosamine-binding hemagglutinin from Parkia platycephala seeds., Cavada BS, Moreno FB, da Rocha BA, de Azevedo WF Jr, Castellon RE, Goersch GV, Nagano CS, de Souza EP, Nascimento KS, Radis-Baptista G, Delatorre P, Leroy Y, Toyama MH, Pinto VP, Sampaio AH, Barettino D, Debray H, Calvete JJ, Sanz L, FEBS J. 2006 Sep;273(17):3962-74. PMID:16934035 Page seeded by OCA on Sun May 4 05:28:35 2008

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