2gv5
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2gv5.gif|left|200px]] | [[Image:2gv5.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2gv5", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2gv5| PDB=2gv5 | SCENE= }} | |
| - | | | + | |
| - | | | + | |
| - | }} | + | |
'''crystal structure of Sfi1p/Cdc31p complex''' | '''crystal structure of Sfi1p/Cdc31p complex''' | ||
| Line 31: | Line 28: | ||
[[Category: Sandercock, A M.]] | [[Category: Sandercock, A M.]] | ||
[[Category: Williams, R L.]] | [[Category: Williams, R L.]] | ||
| - | [[Category: | + | [[Category: Cdc31p]] |
| - | [[Category: | + | [[Category: Centrin]] |
| - | [[Category: | + | [[Category: Centrosome]] |
| - | [[Category: | + | [[Category: Sfi1p]] |
| - | [[Category: | + | [[Category: Spindle pole body]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:34:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:34, 4 May 2008
crystal structure of Sfi1p/Cdc31p complex
Overview
Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly.
About this Structure
2GV5 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication., Li S, Sandercock AM, Conduit P, Robinson CV, Williams RL, Kilmartin JV, J Cell Biol. 2006 Jun 19;173(6):867-77. PMID:16785321 Page seeded by OCA on Sun May 4 05:34:36 2008
