2gxa

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[[Image:2gxa.gif|left|200px]]
[[Image:2gxa.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2gxa |SIZE=350|CAPTION= <scene name='initialview01'>2gxa</scene>, resolution 3.15&Aring;
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The line below this paragraph, containing "STRUCTURE_2gxa", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= E1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10559 Bovine papillomavirus type 1])
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-->
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|DOMAIN=
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{{STRUCTURE_2gxa| PDB=2gxa | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gxa OCA], [http://www.ebi.ac.uk/pdbsum/2gxa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gxa RCSB]</span>
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}}
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'''Crystal structure of papillomavirus E1 hexameric helicase with ssDNA and MgADP'''
'''Crystal structure of papillomavirus E1 hexameric helicase with ssDNA and MgADP'''
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[[Category: Enemark, E J.]]
[[Category: Enemark, E J.]]
[[Category: Joshua-Tor, L.]]
[[Category: Joshua-Tor, L.]]
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[[Category: aaa+]]
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[[Category: Aaa+]]
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[[Category: atpase]]
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[[Category: Atpase]]
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[[Category: dna helicase]]
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[[Category: Dna helicase]]
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[[Category: initiator protein]]
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[[Category: Initiator protein]]
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[[Category: replication]]
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[[Category: Replication]]
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[[Category: virus]]
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[[Category: Virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:38:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:22:53 2008''
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Revision as of 02:38, 4 May 2008

Image:2gxa.gif

Template:STRUCTURE 2gxa

Crystal structure of papillomavirus E1 hexameric helicase with ssDNA and MgADP


Overview

The E1 protein of papillomavirus is a hexameric ring helicase belonging to the AAA + family. The mechanism that couples the ATP cycle to DNA translocation has been unclear. Here we present the crystal structure of the E1 hexamer with single-stranded DNA discretely bound within the hexamer channel and nucleotides at the subunit interfaces. This structure demonstrates that only one strand of DNA passes through the hexamer channel and that the DNA-binding hairpins of each subunit form a spiral 'staircase' that sequentially tracks the oligonucleotide backbone. Consecutively grouped ATP, ADP and apo configurations correlate with the height of the hairpin, suggesting a straightforward DNA translocation mechanism. Each subunit sequentially progresses through ATP, ADP and apo states while the associated DNA-binding hairpin travels from the top staircase position to the bottom, escorting one nucleotide of single-stranded DNA through the channel. These events permute sequentially around the ring from one subunit to the next.

About this Structure

2GXA is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.

Reference

Mechanism of DNA translocation in a replicative hexameric helicase., Enemark EJ, Joshua-Tor L, Nature. 2006 Jul 20;442(7100):270-5. PMID:16855583 Page seeded by OCA on Sun May 4 05:38:38 2008

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