2h0e
From Proteopedia
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'''Crystal Structure of PucM in the absence of substrate''' | '''Crystal Structure of PucM in the absence of substrate''' | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Rhee, S.]] | [[Category: Rhee, S.]] | ||
| - | [[Category: | + | [[Category: Beta sandwitch]] |
| - | [[Category: | + | [[Category: Hiu]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:43:14 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:43, 4 May 2008
Crystal Structure of PucM in the absence of substrate
Overview
The ureide pathway, which produces ureides from uric acid, is an essential purine catabolic process for storing and transporting the nitrogen fixed in leguminous plants and some bacteria. PucM from Bacillus subtilis was recently characterized and found to catalyze the second reaction of the pathway, hydrolyzing 5-hydroxyisourate (HIU), a product of uricase in the first step. PucM has 121 amino acid residues and shows high sequence similarity to the functionally unrelated protein transthyretin (TTR), a thyroid hormone-binding protein. Therefore, PucM belongs to the TTR-related proteins (TRP) family. The crystal structures of PucM at 2.0 A and its complexes with the substrate analogs 8-azaxanthine and 5,6-diaminouracil reveal that even with their overall structure similarity, homotetrameric PucM and TTR are completely different, both in their electrostatic potential and in the size of the active sites located at the dimeric interface. Nevertheless, the absolutely conserved residues across the TRP family, including His-14, Arg-49, His-105, and the C-terminal Tyr-118-Arg-119-Gly-120-Ser-121, indeed form the active site of PucM. Based on the results of site-directed mutagenesis of these residues, we propose a possible mechanism for HIU hydrolysis. The PucM structure determined for the TRP family leads to the conclusion that diverse members of the TRP family would function similarly to PucM as HIU hydrolase.
About this Structure
2H0E is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Structural and functional analysis of PucM, a hydrolase in the ureide pathway and a member of the transthyretin-related protein family., Jung DK, Lee Y, Park SG, Park BC, Kim GH, Rhee S, Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):9790-5. Epub 2006 Jun 16. PMID:16782815 Page seeded by OCA on Sun May 4 05:43:14 2008
