This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2h0s
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2h0s.gif|left|200px]] | [[Image:2h0s.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2h0s", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_2h0s| PDB=2h0s | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme''' | '''Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2H0S is a [[Single protein]] structure | + | 2H0S is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H0S OCA]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 23: | ||
[[Category: Amare, A R.Ferre-D.]] | [[Category: Amare, A R.Ferre-D.]] | ||
[[Category: Klein, D J.]] | [[Category: Klein, D J.]] | ||
| - | [[Category: | + | [[Category: Helix]] |
| - | [[Category: | + | [[Category: Pseudoknot]] |
| - | [[Category: | + | [[Category: Ribozyme]] |
| - | [[Category: | + | [[Category: Rna]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:44:02 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:44, 4 May 2008
Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme
Overview
The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.
About this Structure
2H0S is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:16990543 Page seeded by OCA on Sun May 4 05:44:02 2008
Categories: Single protein | Amare, A R.Ferre-D. | Klein, D J. | Helix | Pseudoknot | Ribozyme | Rna
