2h34

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2h34.gif|left|200px]]
[[Image:2h34.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2h34 |SIZE=350|CAPTION= <scene name='initialview01'>2h34</scene>, resolution 2.800&Aring;
+
The line below this paragraph, containing "STRUCTURE_2h34", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= pknE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2h34| PDB=2h34 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h34 OCA], [http://www.ebi.ac.uk/pdbsum/2h34 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h34 RCSB]</span>
+
-
}}
+
'''Apoenzyme crystal structure of the tuberculosis serine/threonine kinase, PknE'''
'''Apoenzyme crystal structure of the tuberculosis serine/threonine kinase, PknE'''
Line 29: Line 26:
[[Category: Gay, L M.]]
[[Category: Gay, L M.]]
[[Category: Ng, H L.]]
[[Category: Ng, H L.]]
-
[[Category: apoenzyme]]
+
[[Category: Apoenzyme]]
-
[[Category: serine/threonine protein kinase]]
+
[[Category: Serine/threonine protein kinase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:48:44 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:24:47 2008''
+

Revision as of 02:48, 4 May 2008

Image:2h34.gif

Template:STRUCTURE 2h34

Apoenzyme crystal structure of the tuberculosis serine/threonine kinase, PknE


Overview

The "eukaryotic-like" receptor Ser/Thr protein kinases (STPKs) are candidates for the sensors that mediate environmental adaptations of Mycobacterium tuberculosis (Mtb). To define the mechanisms of regulation and substrate recognition, we determined the crystal structure of the ligand-free, activated kinase domain (KD) of the Mtb STPK, PknE. Remarkably, the PknE KD formed a dimer similar to that first observed in the structure of the ATPgammaS complex of the Mtb paralog, PknB. This structural similarity, which occurs despite little sequence conservation between the PknB and PknE dimer interfaces, supports the idea that dimerization regulates the Mtb receptor STPKs. Insertion of the DFG motif into the ATP-binding site and other conformational differences compared the ATPgammaS:PknB complex suggest that apo-PknE is not pre-organized to bind nucleotides. This structure may represent an inactive conformation stabilized by dimerization or, alternatively, an active conformation that reveals shifts that mediate nucleotide exchange and order substrate binding.

About this Structure

2H34 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

A conserved dimer and global conformational changes in the structure of apo-PknE Ser/Thr protein kinase from Mycobacterium tuberculosis., Gay LM, Ng HL, Alber T, J Mol Biol. 2006 Jul 7;360(2):409-20. Epub 2006 May 19. PMID:16762364 Page seeded by OCA on Sun May 4 05:48:44 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2h34"
Personal tools