2h43
From Proteopedia
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'''Crystal Structure of Human Fragment D Complexed with Ala-His-Arg-Pro-amide''' | '''Crystal Structure of Human Fragment D Complexed with Ala-His-Arg-Pro-amide''' | ||
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[[Category: Doolittle, R F.]] | [[Category: Doolittle, R F.]] | ||
[[Category: Pandi, L.]] | [[Category: Pandi, L.]] | ||
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| - | [[Category: | + | [[Category: Fragment d]] |
| - | [[Category: | + | [[Category: Knob-hole interaction]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:50:38 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:50, 4 May 2008
Crystal Structure of Human Fragment D Complexed with Ala-His-Arg-Pro-amide
Overview
The beta-chain amino-terminal sequences of all known mammalian fibrins begin with the sequence Gly-His-Arg-Pro- (GHRP-), but the homologous sequence in chicken fibrin begins with the sequence Ala-His-Arg-Pro- (AHRP-). Nonetheless, chicken fibrinogen binds the synthetic peptide GHRPam, and a previously reported crystal structure has revealed that the binding is in exact conformance with that observed for the human GHRPam-fragment D complex. We now report that human fibrinogen, which is known not to bind APRP, binds the synthetic peptide AHRPam. Moreover, a crystal structure of AHRPam complexed with fragment D from human fibrinogen shows that AHRPam binds exclusively to the beta-chain hole and, unlike GHRPam, not at all to the homologous gamma-chain hole. The difference can be attributed to the methyl group of the alanine residue clashing with a critical carboxyl group in the gammaC hole but being accommodated in the roomier betaC hole where the equivalent carboxyl is situated more flexibly.
About this Structure
2H43 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Differences in binding specificity for the homologous gamma- and beta-chain "holes" on fibrinogen: exclusive binding of Ala-His-Arg-Pro-amide by the beta-chain hole., Doolittle RF, Chen A, Pandi L, Biochemistry. 2006 Nov 28;45(47):13962-9. PMID:17115691 Page seeded by OCA on Sun May 4 05:50:38 2008
