2h6b
From Proteopedia
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'''Crystal structure of oxidized CprK in complex with o-chlorophenolacetic acid''' | '''Crystal structure of oxidized CprK in complex with o-chlorophenolacetic acid''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H6B OCA]. | |
==Reference== | ==Reference== | ||
CprK crystal structures reveal mechanism for transcriptional control of halorespiration., Joyce MG, Levy C, Gabor K, Pop SM, Biehl BD, Doukov TI, Ryter JM, Mazon H, Smidt H, van den Heuvel RH, Ragsdale SW, van der Oost J, Leys D, J Biol Chem. 2006 Sep 22;281(38):28318-25. Epub 2006 Jun 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16803881 16803881] | CprK crystal structures reveal mechanism for transcriptional control of halorespiration., Joyce MG, Levy C, Gabor K, Pop SM, Biehl BD, Doukov TI, Ryter JM, Mazon H, Smidt H, van den Heuvel RH, Ragsdale SW, van der Oost J, Leys D, J Biol Chem. 2006 Sep 22;281(38):28318-25. Epub 2006 Jun 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16803881 16803881] | ||
| - | [[Category: Desulfitobacterium hafniense]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Joyce, M G.]] | [[Category: Joyce, M G.]] | ||
[[Category: Levy, C.]] | [[Category: Levy, C.]] | ||
[[Category: Leys, D.]] | [[Category: Leys, D.]] | ||
| - | [[Category: | + | [[Category: Chlorinated ligand]] |
| - | [[Category: | + | [[Category: Chlorophenol]] |
| - | [[Category: | + | [[Category: Cprk]] |
| - | [[Category: | + | [[Category: Dna binding]] |
| - | [[Category: | + | [[Category: Halorespiration]] |
| - | [[Category: | + | [[Category: Helix-turn-helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:55:04 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:55, 4 May 2008
Crystal structure of oxidized CprK in complex with o-chlorophenolacetic acid
Overview
Halorespiration is a bacterial respiratory process in which haloorganic compounds act as terminal electron acceptors. This process is controlled at transcriptional level by CprK, a member of the ubiquitous CRP-FNR family. Here we present the crystal structures of oxidized CprK in presence of the ligand ortho-chlorophenolacetic acid and of reduced CprK in absence of this ligand. These structures reveal that highly specific binding of chlorinated, rather than the corresponding non-chlorinated, phenolic compounds in the NH(2)-terminal beta-barrels causes reorientation of these domains with respect to the central alpha-helix at the dimer interface. Unexpectedly, the COOH-terminal DNA-binding domains dimerize in the non-DNA binding state. We postulate the ligand-induced conformational change allows formation of interdomain contacts that disrupt the DNA domain dimer interface and leads to repositioning of the helix-turn-helix motifs. These structures provide a structural framework for further studies on transcriptional control by CRP-FNR homologs in general and of halorespiration regulation by CprK in particular.
About this Structure
Full crystallographic information is available from OCA.
Reference
CprK crystal structures reveal mechanism for transcriptional control of halorespiration., Joyce MG, Levy C, Gabor K, Pop SM, Biehl BD, Doukov TI, Ryter JM, Mazon H, Smidt H, van den Heuvel RH, Ragsdale SW, van der Oost J, Leys D, J Biol Chem. 2006 Sep 22;281(38):28318-25. Epub 2006 Jun 27. PMID:16803881 Page seeded by OCA on Sun May 4 05:55:04 2008
