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2h95
From Proteopedia
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[[Image:2h95.jpg|left|200px]] | [[Image:2h95.jpg|left|200px]] | ||
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'''Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy''' | '''Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2H95 is a [[Single protein]] structure | + | 2H95 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H95 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cross, T A.]] | [[Category: Cross, T A.]] | ||
[[Category: Hu, J.]] | [[Category: Hu, J.]] | ||
| - | [[Category: | + | [[Category: Alpha helix]] |
| - | [[Category: | + | [[Category: Protein-ligand]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:00:39 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:00, 4 May 2008
Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy
Overview
Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates a kink in the monomer between two helical fragments having 20 degrees and 31 degrees tilt angles with respect to the membrane normal. This monomer structure is then used to construct a plausible model of the tetrameric amantadine-blocked M2 trans-membrane channel. The influence of amantadine binding through comparative cross polarization magic-angle spinning spectra was also observed. In addition, spectra are shown of the amantadine-resistant mutant, S31N, in the presence and absence of amantadine.
About this Structure
2H95 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus., Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:17384070 Page seeded by OCA on Sun May 4 06:00:39 2008
