2hb5
From Proteopedia
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'''Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain''' | '''Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain''' | ||
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[[Category: Lim, D.]] | [[Category: Lim, D.]] | ||
[[Category: Martinez-Hackert, E.]] | [[Category: Martinez-Hackert, E.]] | ||
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Revision as of 03:04, 4 May 2008
Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain
Overview
A crystallographic study of the Moloney murine leukemia virus (Mo-MLV) RNase H domain was performed to provide information about its structure and mechanism of action. These efforts resulted in the crystallization of a mutant Mo-MLV RNase H lacking the putative helix C (DeltaC). The 1.6-Angstroms resolution structure resembles the known structures of the human immunodeficiency virus type 1 (HIV-1) and Escherichia coli RNase H. The structure revealed the coordination of a magnesium ion within the catalytic core comprised of the highly conserved acidic residues D524, E562, and D583. Surface charge mapping of the Mo-MLV structure revealed a high density of basic charges on one side of the enzyme. Using a model of the Mo-MLV structure superimposed upon a structure of HIV-1 reverse transcriptase bound to an RNA/DNA hybrid substrate, Mo-MLV RNase H secondary structures and individual amino acids were examined for their potential roles in binding substrate. Identified regions included Mo-MLV RNase H beta1-beta2, alphaA, and alphaB and residues from alphaB to alphaD and its following loop. Most of the identified substrate-binding residues corresponded with residues directly binding nucleotides in an RNase H from Bacillus halodurans as observed in a cocrystal structure with RNA/DNA. Finally, superimposition of RNases H of Mo-MLV, E. coli, and HIV-1 revealed that a loop of the HIV-1 connection domain resides within the same region of the Mo-MLV and E. coli C-helix. The HIV-1 connection domain may serve to recognize and bind the RNA/DNA substrate major groove.
About this Structure
2HB5 is a Single protein structure of sequence from Moloney murine leukemia virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the moloney murine leukemia virus RNase H domain., Lim D, Gregorio GG, Bingman C, Martinez-Hackert E, Hendrickson WA, Goff SP, J Virol. 2006 Sep;80(17):8379-89. PMID:16912289 Page seeded by OCA on Sun May 4 06:04:49 2008
