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2p2r

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(New page: 200px<br /> <applet load="2p2r" size="450" color="white" frame="true" align="right" spinBox="true" caption="2p2r, resolution 1.60&Aring;" /> '''Crystal structure o...)
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Revision as of 21:13, 12 November 2007

Image:2p2r.gif

2p2r, resolution 1.60Å

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Crystal structure of the third KH domain of human Poly(C)-Binding Protein-2 in complex with C-rich strand of human telomeric DNA

Overview

KH (hnRNP K homology) domains, consisting of approximately 70 amino acid, residues, are present in a variety of nucleic-acid-binding proteins. Among, these are poly(C)-binding proteins (PCBPs), which are important regulators, of mRNA stability and posttranscriptional regulation in general. All PCBPs, contain three different KH domains and recognize poly(C)-sequences with, high affinity and specificity. To reveal the molecular basis of, poly(C)-sequence recognition, we have determined the crystal structure, at, 1.6 A resolution, of PCBP2 KH3 domain in complex with a 7-nt DNA sequence, (5'-AACCCTA-3') corresponding to one repeat of the C-rich strand of human, telomeric DNA. The domain assumes a type-I KH fold in a, betaalphaalphabetabetaalpha configuration. The protein-DNA interface could, be studied in unprecedented detail and is made up of a series of direct, and water-mediated hydrogen bonds between the protein and the DNA, revealing an especially dense network involving several structural water, molecules for the last 2 nt in the core recognition sequence. Unlike, published KH domain structures, the protein crystallizes without, protein-protein contacts, yielding new insights into the dimerization, properties of different KH domains. A nucleotide platform, an interesting, feature found in some RNA molecules, was identified, evidently for the, first time in DNA.

About this Structure

2P2R is a Single protein structure of sequence from Homo sapiens with CYT as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution., Fenn S, Du Z, Lee JK, Tjhen R, Stroud RM, James TL, Nucleic Acids Res. 2007;35(8):2651-60. Epub 2007 Apr 10. PMID:17426136

Page seeded by OCA on Mon Nov 12 23:20:02 2007

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