Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2hl5
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2hl5.gif|left|200px]] | [[Image:2hl5.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2hl5", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2hl5| PDB=2hl5 | SCENE= }} | |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''Crystal structure of the C-terminal domain of human EB1 in complex with the A49M mutant CAP-Gly domain of human Dynactin-1 (p150-Glued)''' | '''Crystal structure of the C-terminal domain of human EB1 in complex with the A49M mutant CAP-Gly domain of human Dynactin-1 (p150-Glued)''' | ||
| Line 32: | Line 29: | ||
[[Category: Winkler, F K.]] | [[Category: Winkler, F K.]] | ||
[[Category: +tip protein complex structure]] | [[Category: +tip protein complex structure]] | ||
| - | [[Category: | + | [[Category: Coiled coil]] |
| - | [[Category: | + | [[Category: Cytoskeleton associated protein]] |
| - | [[Category: | + | [[Category: Dynactin]] |
| - | [[Category: | + | [[Category: Eb1]] |
| - | [[Category: | + | [[Category: Microtubule binding]] |
| - | [[Category: | + | [[Category: P150glued]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:25:08 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:25, 4 May 2008
Crystal structure of the C-terminal domain of human EB1 in complex with the A49M mutant CAP-Gly domain of human Dynactin-1 (p150-Glued)
Contents |
Overview
Dynamic microtubule plus-end tracking protein (+TIP) networks are implicated in all functions of microtubules, but the molecular determinants of their interactions are largely unknown. Here, we have explored key binding modes of +TIPs by analyzing the interactions between selected CAP-Gly, EB-like, and carboxy-terminal EEY/F-COO(-) sequence motifs. X-ray crystallography and biophysical binding studies demonstrate that the beta2-beta3 loop of CAP-Gly domains determines EB-like motif binding specificity. They further show how CAP-Gly domains serve as recognition domains for EEY/F-COO(-) motifs, which represent characteristic and functionally important sequence elements in EB, CLIP-170, and alpha-tubulin. Our findings provide a molecular basis for understanding the modular interaction modes between alpha-tubulin, CLIPs, EB proteins, and the dynactin-dynein motor complex and suggest that multiple low-affinity binding sites in different combinations control dynamic +TIP networks at microtubule ends. They further offer insights into the structural consequences of genetic CAP-Gly domain defects found in severe human disorders.
Disease
Known disease associated with this structure: Neuropathy, distal hereditary motor, type VIIB OMIM:[601143], Amyotrophic lateral sclerosis, susceptibility to OMIM:[601143]
About this Structure
2HL5 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Key interaction modes of dynamic +TIP networks., Honnappa S, Okhrimenko O, Jaussi R, Jawhari H, Jelesarov I, Winkler FK, Steinmetz MO, Mol Cell. 2006 Sep 1;23(5):663-71. PMID:16949363 Page seeded by OCA on Sun May 4 06:25:08 2008
