2hl7
From Proteopedia
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'''Crystal structure of the periplasmic domain of CcmH from Pseudomonas aeruginosa''' | '''Crystal structure of the periplasmic domain of CcmH from Pseudomonas aeruginosa''' | ||
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[[Category: Three-helices bundle]] | [[Category: Three-helices bundle]] | ||
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Revision as of 03:25, 4 May 2008
Crystal structure of the periplasmic domain of CcmH from Pseudomonas aeruginosa
Overview
CcmH (cytochromes c maturation protein H) is an essential component of the assembly line necessary for the maturation of c-type cytochromes in the periplasm of Gram-negative bacteria. The protein is a membrane-anchored thiol-oxidoreductase that has been hypothesized to be involved in the recognition and reduction of apocytochrome c, a prerequisite for covalent heme attachment. Here, we present the 1.7A crystal structure of the soluble periplasmic domain of CcmH from the opportunistic pathogen Pseudomonas aeruginosa (Pa-CcmH*). The protein contains a three-helix bundle, i.e. a fold that is different from that of all other thiol-oxidoreductases reported so far. The catalytic Cys residues of the conserved LRCXXC motif (Cys(25) and Cys(28)), located in a long loop connecting the first two helices, form a disulfide bond in the oxidized enzyme. We have determined the pK(a) values of these 2 Cys residues of Pa-CcmH* (both >8) and propose a possible mechanistic role for a conserved Ser(36) and a water molecule in the active site. The interaction between Pa-CcmH* and Pa-apocyt c(551) (where cyt c(551) represents cytochrome c(551)) was characterized in vitro following the binding kinetics by stopped-flow using a Trp-containing fluorescent variant of Pa-CcmH* and a dansylated peptide, mimicking the apocytochrome c(551) heme binding motif. The kinetic results show that the protein has a moderate affinity to its apocyt substrate, consistent with the role of Pa-CcmH as an intermediate component of the assembly line for c-type cytochrome biogenesis.
About this Structure
2HL7 is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
A strategic protein in cytochrome c maturation: three-dimensional structure of CcmH and binding to apocytochrome c., Di Matteo A, Gianni S, Schinina ME, Giorgi A, Altieri F, Calosci N, Brunori M, Travaglini-Allocatelli C, J Biol Chem. 2007 Sep 14;282(37):27012-9. Epub 2007 Jul 10. PMID:17623665 Page seeded by OCA on Sun May 4 06:25:13 2008
