2hlv
From Proteopedia
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[[Image:2hlv.jpg|left|200px]] | [[Image:2hlv.jpg|left|200px]] | ||
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'''Bovine Odorant Binding Protein deswapped triple mutant''' | '''Bovine Odorant Binding Protein deswapped triple mutant''' | ||
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[[Category: Spinelli, S.]] | [[Category: Spinelli, S.]] | ||
[[Category: Tegoni, M.]] | [[Category: Tegoni, M.]] | ||
- | [[Category: | + | [[Category: Domain swapping]] |
- | [[Category: | + | [[Category: Odorant binding protein]] |
- | [[Category: | + | [[Category: Transport protein]] |
- | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:26:33 2008'' | |
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + |
Revision as of 03:26, 4 May 2008
Bovine Odorant Binding Protein deswapped triple mutant
Overview
The X-ray structure of bovine Odorant Binding Protein (bOBP) revealed its association as a domain swapped dimer. bOBP, devoid of any cysteines, contrasts with other mammalian OBPs, which are monomeric and possess at least one disulfide bridge. We have produced a mutant of bOBP in which a glycine residue was inserted after position 121. This mutation yielded a monomeric bOBP-121Gly+ in which domain swapping has been reverted. Here, we have subsequently introduced two mutations, Trp64Cys and His155Cys, in view to stabilize the putative monomer with a disulfide bridge. We have determined the crystal structure of this triple mutant at 1.65 A resolution. The mutant protein is monomeric, stabilized by a disulfide bridge between Trp64Cys and His155Cys, with a backbone superimposable to that of native bOBP, with the exception of the hinge and of the 10 residues at the C-terminus. bOBP triple mutant binds 1-amino-anthracene, 1-octen-3-ol (bOBP co-purified ligand) and other ligands with muM Kd values comparable to those of the swapped dimer.
About this Structure
2HLV is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Deswapping bovine odorant binding protein., Ramoni R, Spinelli S, Grolli S, Conti V, Merli E, Cambillau C, Tegoni M, Biochim Biophys Acta. 2008 Apr;1784(4):651-7. Epub 2008 Jan 29. PMID:18269920 Page seeded by OCA on Sun May 4 06:26:33 2008