2hos

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[[Image:2hos.gif|left|200px]]
[[Image:2hos.gif|left|200px]]
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{{Structure
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|PDB= 2hos |SIZE=350|CAPTION= <scene name='initialview01'>2hos</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_2hos", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=3MO:3-METHYL-1,3-OXAZOLIDIN-2-ONE'>3MO</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE= en ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])
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|DOMAIN=
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{{STRUCTURE_2hos| PDB=2hos | SCENE= }}
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|RELATEDENTRY=[[2hdd|2HDD]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [http://www.ebi.ac.uk/pdbsum/2hos PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB]</span>
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}}
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'''Phage-Selected Homeodomain Bound to Unmodified DNA'''
'''Phage-Selected Homeodomain Bound to Unmodified DNA'''
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[[Category: Shokat, K M.]]
[[Category: Shokat, K M.]]
[[Category: Simon, M D.]]
[[Category: Simon, M D.]]
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[[Category: homeodomain]]
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[[Category: Homeodomain]]
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[[Category: phage display]]
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[[Category: Phage display]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:31:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:33:12 2008''
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Revision as of 03:31, 4 May 2008

Image:2hos.gif

Template:STRUCTURE 2hos

Phage-Selected Homeodomain Bound to Unmodified DNA


Overview

The homeodomain (HD)-DNA interface has been conserved over 500 million years of evolution. Despite this conservation, we have successfully re-engineered the engrailed HD to specifically recognize an unnatural nucleotide using a phage display selection. Here we report the synthesis of novel nucleosides and the selection of mutant HDs that bind these nucleotides using phage display. The high-resolution crystal structure of one mutant in complex with modified and unmodified DNA demonstrates that, even with the substantial perturbation to the interface, this selected mutant retains a canonical HD structure. Dissection of the contributions due to each of the selected mutations reveals that the majority of the modification-specific binding is accomplished by a single mutation (I47G) but that the remaining mutations retune the stability of the HD. These results afford a detailed look at a re-engineered protein-DNA interaction and provide insight into the opportunities for re-engineering highly conserved interfaces.

About this Structure

2HOS is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Structure and properties of a re-engineered homeodomain protein-DNA interface., Simon MD, Feldman ME, Rauh D, Maris AE, Wemmer DE, Shokat KM, ACS Chem Biol. 2006 Dec 15;1(12):755-60. PMID:17240973 Page seeded by OCA on Sun May 4 06:31:34 2008

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OCA

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