2hqs

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[[Image:2hqs.jpg|left|200px]]
[[Image:2hqs.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2hqs |SIZE=350|CAPTION= <scene name='initialview01'>2hqs</scene>, resolution 1.50&Aring;
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The line below this paragraph, containing "STRUCTURE_2hqs", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= tolB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), pal, excC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_2hqs| PDB=2hqs | SCENE= }}
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|RELATEDENTRY=[[1c5k|1C5K]], [[1oap|1OAP]], [[1crz|1CRZ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hqs OCA], [http://www.ebi.ac.uk/pdbsum/2hqs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hqs RCSB]</span>
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}}
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'''Crystal structure of TolB/Pal complex'''
'''Crystal structure of TolB/Pal complex'''
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[[Category: Grishkovskaya, I.]]
[[Category: Grishkovskaya, I.]]
[[Category: Kleanthous, C.]]
[[Category: Kleanthous, C.]]
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[[Category: pal]]
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[[Category: Pal]]
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[[Category: tol]]
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[[Category: Tol]]
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[[Category: tolb]]
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[[Category: Tolb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:35:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:34:05 2008''
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Revision as of 03:35, 4 May 2008

Image:2hqs.jpg


PDB ID 2hqs

Drag the structure with the mouse to rotate
2hqs, resolution 1.50Å ()
Ligands: , ,
Gene: tolB (Escherichia coli), pal, excC (Escherichia coli)
Related: 1c5k, 1oap, 1crz
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



Crystal structure of TolB/Pal complex


Overview

We report the crystal structure of the Escherichia coli TolB-Pal complex, a protein-protein complex involved in maintaining the integrity of the outer membrane (OM) in all Gram-negative bacteria that is parasitized by colicins (protein antibiotics) to expedite their entry into cells. Nuclease colicins competitively recruit TolB using their natively disordered regions (NDRs) to disrupt its complex with Pal, which is thought to trigger translocation of the toxin across a locally destabilized OM. The structure shows induced-fit binding of peptidoglycan-associated lipoprotein (Pal) to the beta-propeller domain of TolB causing the N-terminus of one of its alpha-helices to unwind and several residues to undergo substantial changes in conformation. The resulting interactions with TolB are known to be essential for the stability of the complex and the bacterial OM. Structural comparisons with a TolB-colicin NDR complex reveal that colicins bind at the Pal site, mimicking rearranged Pal residues while simultaneously appearing to block induced-fit changes in TolB. The study therefore explains how colicins recruit TolB in the bacterial periplasm and highlights a novel binding mechanism for a natively disordered protein.

About this Structure

2HQS is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Molecular mimicry enables competitive recruitment by a natively disordered protein., Bonsor DA, Grishkovskaya I, Dodson EJ, Kleanthous C, J Am Chem Soc. 2007 Apr 18;129(15):4800-7. Epub 2007 Mar 22. PMID:17375930 Page seeded by OCA on Sun May 4 06:35:49 2008

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