2px9
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(New page: 200px<br /> <applet load="2px9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2px9" /> '''The intrinsic affinity between E2 and the C...)
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Revision as of 21:21, 12 November 2007
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The intrinsic affinity between E2 and the Cys domain of E1 in Ubiquitin-like modifications
Overview
Ubiquitin-like modifications, which are carried out by similar biochemical, mechanisms, regulate nearly every aspect of cellular function. Despite the, recent advancements in characterizing their enzymology, our knowledge, about the dynamic processes of these modifications is still fragmentary., In this study, we have uncovered an intrinsic affinity between the SUMO E2, and the Cys domain of SUMO E1. NMR studies in combination with, paramagnetic spin labeling demonstrate that this interaction is mediated, by previously unknown interfaces on both E1 and E2 and places the two, catalytic Cys residues of the two enzymes in close proximity., Site-directed mutagenesis and enzymatic assays indicate that the, interaction is fundamentally important for the transfer of SUMO from E1 to, E2. Results from this study suggest that the interaction between E2 and, the Cys domain of E1 participates in guiding the E2's translocation to, E1's enzymatic active site in ubiquitin-like modifications.
About this Structure
2PX9 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The intrinsic affinity between E2 and the Cys domain of E1 in ubiquitin-like modifications., Wang J, Hu W, Cai S, Lee B, Song J, Chen Y, Mol Cell. 2007 Jul 20;27(2):228-37. PMID:17643372
Page seeded by OCA on Mon Nov 12 23:27:50 2007
Categories: Homo sapiens | Protein complex | Cai, S. | Chen, Y. | Hu, W.D. | Lee, B. | Song, J. | Wang, J.H. | E1 | E2 | Nmr | Paramagnetic spin-labeling | Protein-protein interaction | Sae2 | Sumo | Ubc9 | Ubiquitination
