2hza

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[[Image:2hza.gif|left|200px]]
[[Image:2hza.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2hza |SIZE=350|CAPTION= <scene name='initialview01'>2hza</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_2hza", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=3CM:3-CYCLOHEXYLPROPYL+4-O-ALPHA-D-GLUCOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE'>3CM</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= nikR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_2hza| PDB=2hza | SCENE= }}
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|RELATEDENTRY=[[1q5y|1Q5Y]], [[1q5v|1Q5V]], [[2hzv|2HZV]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hza OCA], [http://www.ebi.ac.uk/pdbsum/2hza PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hza RCSB]</span>
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}}
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'''Nickel-bound full-length Escherichia coli NikR'''
'''Nickel-bound full-length Escherichia coli NikR'''
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[[Category: Drennan, C L.]]
[[Category: Drennan, C L.]]
[[Category: Schreiter, E R.]]
[[Category: Schreiter, E R.]]
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[[Category: nickel-binding]]
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[[Category: Nickel-binding]]
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[[Category: ribbon-helix-helix]]
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[[Category: Ribbon-helix-helix]]
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[[Category: transcription factor]]
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[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:53:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:37:32 2008''
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Revision as of 03:53, 4 May 2008

Image:2hza.gif

Template:STRUCTURE 2hza

Nickel-bound full-length Escherichia coli NikR


Overview

Metal ion homeostasis is critical to the survival of all cells. Regulation of nickel concentrations in Escherichia coli is mediated by the NikR repressor via nickel-induced transcriptional repression of the nickel ABC-type transporter, NikABCDE. Here, we report two crystal structures of nickel-activated E. coli NikR, the isolated repressor at 2.1 A resolution and in a complex with its operator DNA sequence from the nik promoter at 3.1 A resolution. Along with the previously published structure of apo-NikR, these structures allow us to evaluate functional proposals for how metal ions activate NikR, delineate the drastic conformational changes required for operator recognition, and describe the formation of a second metal-binding site in the presence of DNA. They also provide a rare set of structural views of a ligand-responsive transcription factor in the unbound, ligand-induced, and DNA-bound states, establishing a model system for the study of ligand-mediated effects on transcription factor function.

About this Structure

2HZA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

NikR-operator complex structure and the mechanism of repressor activation by metal ions., Schreiter ER, Wang SC, Zamble DB, Drennan CL, Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13676-81. Epub 2006 Aug 31. PMID:16945905 Page seeded by OCA on Sun May 4 06:53:37 2008

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