2hzc
From Proteopedia
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[[Image:2hzc.gif|left|200px]] | [[Image:2hzc.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_2hzc| PDB=2hzc | SCENE= }} | |
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'''Crystal structure of the N-terminal RRM of the U2AF large subunit''' | '''Crystal structure of the N-terminal RRM of the U2AF large subunit''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HZC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry | + | 2HZC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2fzr 2fzr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Sickmier, E A.]] | [[Category: Sickmier, E A.]] | ||
[[Category: Thickman, K R.]] | [[Category: Thickman, K R.]] | ||
| - | [[Category: | + | [[Category: Alternative conformation]] |
| - | [[Category: | + | [[Category: Rna recognition]] |
| - | [[Category: | + | [[Category: Rna splicing]] |
| - | [[Category: | + | [[Category: Rrm]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:53:44 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:53, 4 May 2008
Crystal structure of the N-terminal RRM of the U2AF large subunit
Overview
The essential pre-mRNA splicing factor, U2 auxiliary factor 65KD (U2AF(65)) recognizes the polypyrimidine tract (Py-tract) consensus sequence of the pre-mRNA using two RNA recognition motifs (RRMs), the most prevalent class of eukaryotic RNA-binding domain. The Py-tracts of higher eukaryotic pre-mRNAs are often interrupted with purines, yet U2AF(65) must identify these degenerate Py-tracts for accurate pre-mRNA splicing. Previously, the structure of a U2AF(65) variant in complex with poly(U) RNA suggested that rearrangement of flexible side-chains or bound water molecules may contribute to degenerate Py-tract recognition by U2AF(65). Here, the X-ray structure of the N-terminal RRM domain of U2AF(65) (RRM1) is described at 1.47 A resolution in the absence of RNA. Notably, RNA-binding by U2AF(65) selectively stabilizes pre-existing alternative conformations of three side-chains located at the RNA interface (Arg150, Lys225, and Arg227). Additionally, a flexible loop connecting the beta2/beta3 strands undergoes a conformational change to interact with the RNA. These pre-existing alternative conformations may contribute to the ability of U2AF(65) to recognize a variety of Py-tract sequences. This rare, high-resolution view of an important member of the RRM class of RNA-binding domains highlights the role of alternative side-chain conformations in RNA recognition.
About this Structure
2HZC is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 2fzr. Full crystallographic information is available from OCA.
Reference
Alternative conformations at the RNA-binding surface of the N-terminal U2AF(65) RNA recognition motif., Thickman KR, Sickmier EA, Kielkopf CL, J Mol Biol. 2007 Feb 23;366(3):703-10. Epub 2006 Dec 2. PMID:17188295 Page seeded by OCA on Sun May 4 06:53:44 2008
