2i3p

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[[Image:2i3p.gif|left|200px]]
[[Image:2i3p.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2i3p |SIZE=350|CAPTION= <scene name='initialview01'>2i3p</scene>, resolution 2.300&Aring;
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The line below this paragraph, containing "STRUCTURE_2i3p", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2i3p| PDB=2i3p | SCENE= }}
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|RELATEDENTRY=[[2i3q|2I3Q]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i3p OCA], [http://www.ebi.ac.uk/pdbsum/2i3p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i3p RCSB]</span>
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}}
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'''K28R mutant of Homing Endonuclease I-CreI'''
'''K28R mutant of Homing Endonuclease I-CreI'''
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[[Category: Rosen, L.]]
[[Category: Rosen, L.]]
[[Category: Sussman, D.]]
[[Category: Sussman, D.]]
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[[Category: dna]]
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[[Category: Dna]]
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[[Category: homing endonulease i-crei]]
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[[Category: Homing endonulease i-crei]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:01:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:39:15 2008''
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Revision as of 04:01, 4 May 2008

Image:2i3p.gif

Template:STRUCTURE 2i3p

K28R mutant of Homing Endonuclease I-CreI


Overview

Homing endonucleases are highly specific enzymes, capable of recognizing and cleaving unique DNA sequences in complex genomes. Since such DNA cleavage events can result in targeted allele-inactivation and/or allele-replacement in vivo, the ability to engineer homing endonucleases matched to specific DNA sequences of interest would enable powerful and precise genome manipulations. We have taken a step-wise genetic approach in analyzing individual homing endonuclease I-CreI protein/DNA contacts, and describe here novel interactions at four distinct target site positions. Crystal structures of two mutant endonucleases reveal the molecular interactions responsible for their altered DNA target specificities. We also combine novel contacts to create an endonuclease with the predicted target specificity. These studies provide important insights into engineering homing endonucleases with novel target specificities, as well as into the evolution of DNA recognition by this fascinating family of proteins.

About this Structure

2I3P is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

Reference

Homing endonuclease I-CreI derivatives with novel DNA target specificities., Rosen LE, Morrison HA, Masri S, Brown MJ, Springstubb B, Sussman D, Stoddard BL, Seligman LM, Nucleic Acids Res. 2006;34(17):4791-800. Epub 2006 Sep 13. PMID:16971456 Page seeded by OCA on Sun May 4 07:01:55 2008

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OCA

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