2i5p
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2i5p.gif|left|200px]] | [[Image:2i5p.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2i5p", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_2i5p| PDB=2i5p | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of glyceraldehyde-3-phosphate dehydrogenase isoform 1 from K. marxianus''' | '''Crystal structure of glyceraldehyde-3-phosphate dehydrogenase isoform 1 from K. marxianus''' | ||
| Line 23: | Line 20: | ||
==Reference== | ==Reference== | ||
The crystal and solution structures of glyceraldehyde-3-phosphate dehydrogenase reveal different quaternary structures., Ferreira-da-Silva F, Pereira PJ, Gales L, Roessle M, Svergun DI, Moradas-Ferreira P, Damas AM, J Biol Chem. 2006 Nov 3;281(44):33433-40. Epub 2006 Sep 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16963457 16963457] | The crystal and solution structures of glyceraldehyde-3-phosphate dehydrogenase reveal different quaternary structures., Ferreira-da-Silva F, Pereira PJ, Gales L, Roessle M, Svergun DI, Moradas-Ferreira P, Damas AM, J Biol Chem. 2006 Nov 3;281(44):33433-40. Epub 2006 Sep 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16963457 16963457] | ||
| - | [[Category: Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)]] | ||
[[Category: Kluyveromyces marxianus]] | [[Category: Kluyveromyces marxianus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 31: | Line 27: | ||
[[Category: Moradas-Ferreira, P.]] | [[Category: Moradas-Ferreira, P.]] | ||
[[Category: Pereira, P J.B.]] | [[Category: Pereira, P J.B.]] | ||
| - | [[Category: | + | [[Category: Apo form]] |
| - | [[Category: | + | [[Category: Dimer]] |
| - | [[Category: | + | [[Category: Gapdh]] |
| - | [[Category: | + | [[Category: Rossmann fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:06:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:06, 4 May 2008
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase isoform 1 from K. marxianus
Overview
The presence of an isoform of glyceraldehyde-3-phosphate dehydrogenase (kmGAPDH1p) associated with the cell wall of a flocculent strain of Kluyveromyces marxianus was the first report of a non-cytosolic localization of a glycolytic enzyme, but the mechanism by which the protein is transported to the cell surface is not known. To identify structural features that could account for the multiple localizations of the protein, the three-dimensional structure of kmGAPDH1p was determined by x-ray crystallography and small angle x-ray scattering. The x-ray crystallographic structure of kmGAPDH1p revealed a dimer, although all GAPDH homologs studied thus far have a tetrameric structure with 222 symmetry. Interestingly, the structure of kmGAPDH1p in solution revealed a tetramer with a 70 degrees tilt angle between the dimers. Moreover, the separation between the centers of the dimers composing the kmGAPDH1p tetramer diminished from 34 to 30 A upon NAD(+) binding, this latter value being similar to the observed in the crystallographic models of GAPDH homologs. The less compact structure of apo-kmGAPDH1p could already be the first image of the transition intermediate between the tetramer observed in solution and the dimeric form found in the crystal structure, which we postulate to exist in vivo because of the protein's multiple subcellular localizations in this yeast species.
About this Structure
2I5P is a Single protein structure of sequence from Kluyveromyces marxianus. Full crystallographic information is available from OCA.
Reference
The crystal and solution structures of glyceraldehyde-3-phosphate dehydrogenase reveal different quaternary structures., Ferreira-da-Silva F, Pereira PJ, Gales L, Roessle M, Svergun DI, Moradas-Ferreira P, Damas AM, J Biol Chem. 2006 Nov 3;281(44):33433-40. Epub 2006 Sep 7. PMID:16963457 Page seeded by OCA on Sun May 4 07:06:22 2008
