2ic1

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[[Image:2ic1.gif|left|200px]]
[[Image:2ic1.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ic1 |SIZE=350|CAPTION= <scene name='initialview01'>2ic1</scene>, resolution 2.7&Aring;
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The line below this paragraph, containing "STRUCTURE_2ic1", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_dioxygenase Cysteine dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.20 1.13.11.20] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= CDO1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2ic1| PDB=2ic1 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ic1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ic1 OCA], [http://www.ebi.ac.uk/pdbsum/2ic1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ic1 RCSB]</span>
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}}
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'''Crystal Structure of Human Cysteine Dioxygenase in Complex with Substrate Cysteine'''
'''Crystal Structure of Human Cysteine Dioxygenase in Complex with Substrate Cysteine'''
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[[Category: Wu, X.]]
[[Category: Wu, X.]]
[[Category: Ye, S.]]
[[Category: Ye, S.]]
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[[Category: cupin]]
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[[Category: Cupin]]
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[[Category: enzyme-substrate complex]]
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[[Category: Enzyme-substrate complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:18:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:42:18 2008''
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Revision as of 04:18, 4 May 2008

Image:2ic1.gif

Template:STRUCTURE 2ic1

Crystal Structure of Human Cysteine Dioxygenase in Complex with Substrate Cysteine


Overview

Cysteine dioxygenase is a non-heme mononuclear iron metalloenzyme that catalyzes the oxidation of cysteine to cysteine sulfinic acid with addition of molecular dioxygen. This irreversible oxidative catabolism of cysteine initiates several important metabolic pathways related to diverse sulfurate compounds. Cysteine dioxygenase is therefore very important for maintaining the proper hepatic concentration of intracellular free cysteine. Mechanisms for mouse and rat cysteine dioxygenases have recently been reported based on their crystal structures in the absence of substrates, although there is still a lack of direct evidence. Here we report the first crystal structure of human cysteine dioxygenase in complex with its substrate L-cysteine to 2.7A, together with enzymatic activity and metal content assays of several single point mutants. Our results provide an insight into a new mechanism of cysteine thiol dioxygenation catalyzed by cysteine dioxygenase, which is tightly associated with a thioether-bonded tyrosine-cysteine cofactor involving Tyr-157 and Cys-93. This cross-linked protein-derived cofactor plays several key roles different from those in galactose oxidase. This report provides a new potential target for therapy of diseases related to human cysteine dioxygenase, including neurodegenerative and autoimmune diseases.

About this Structure

2IC1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

An insight into the mechanism of human cysteine dioxygenase. Key roles of the thioether-bonded tyrosine-cysteine cofactor., Ye S, Wu X, Wei L, Tang D, Sun P, Bartlam M, Rao Z, J Biol Chem. 2007 Feb 2;282(5):3391-402. Epub 2006 Nov 29. PMID:17135237 Page seeded by OCA on Sun May 4 07:18:52 2008

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