2iew

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[[Image:2iew.jpg|left|200px]]
[[Image:2iew.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2iew |SIZE=350|CAPTION= <scene name='initialview01'>2iew</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_2iew", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-polyphosphate_multikinase Inositol-polyphosphate multikinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.151 2.7.1.151] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= IPK2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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-->
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|DOMAIN=
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{{STRUCTURE_2iew| PDB=2iew | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iew OCA], [http://www.ebi.ac.uk/pdbsum/2iew PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iew RCSB]</span>
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}}
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'''Crystal structure of Inositol Phosphate Multikinase Ipk2 from S. cerevisiae'''
'''Crystal structure of Inositol Phosphate Multikinase Ipk2 from S. cerevisiae'''
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[[Category: Holmes, W.]]
[[Category: Holmes, W.]]
[[Category: Jogl, G.]]
[[Category: Jogl, G.]]
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[[Category: atp-grasp fold related]]
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[[Category: Atp-grasp fold related]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:25:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:43:21 2008''
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Revision as of 04:25, 4 May 2008

Image:2iew.jpg

Template:STRUCTURE 2iew

Crystal structure of Inositol Phosphate Multikinase Ipk2 from S. cerevisiae


Overview

Inositol polyphosphates perform essential functions as second messengers in eukaryotic cells, and their cellular levels are regulated by inositol phosphate kinases. Most of these enzymes belong to the inositol phosphate kinase superfamily, which consists of three subgroups, inositol 3-kinases, inositol phosphate multikinases, and inositol hexakisphosphate kinases. Family members share several strictly conserved signature motifs and are expected to have the same backbone fold, despite very limited overall amino acid sequence identity. Sequence differences are expected to play important roles in defining the different substrate selectivity of these enzymes. To investigate the structural basis for substrate specificity, we have determined the crystal structure of the yeast inositol phosphate multikinase Ipk2 in the apoform and in a complex with ADP and Mn(2+) at up to 2.0A resolution. The overall structure of Ipk2 is related to inositol trisphosphate 3-kinase. The ATP binding site is similar in both enzymes; however, the inositol binding domain is significantly smaller in Ipk2. Replacement of critical side chains in the inositolbinding site suggests how modification of substrate recognition motifs determines enzymatic substrate preference and catalysis.

About this Structure

2IEW is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of inositol phosphate multikinase 2 and implications for substrate specificity., Holmes W, Jogl G, J Biol Chem. 2006 Dec 8;281(49):38109-16. Epub 2006 Oct 18. PMID:17050532 Page seeded by OCA on Sun May 4 07:25:29 2008

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