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2ig9

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[[Image:2ig9.gif|left|200px]]
[[Image:2ig9.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ig9 |SIZE=350|CAPTION= <scene name='initialview01'>2ig9</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_2ig9", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3,4-dihydroxyphenylacetate_2,3-dioxygenase 3,4-dihydroxyphenylacetate 2,3-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.15 1.13.11.15] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= hpcd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=47914 Brevibacterium fuscum])
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-->
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|DOMAIN=
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{{STRUCTURE_2ig9| PDB=2ig9 | SCENE= }}
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|RELATEDENTRY=[[2iga|2IGA]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ig9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ig9 OCA], [http://www.ebi.ac.uk/pdbsum/2ig9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ig9 RCSB]</span>
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}}
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'''Structure of a full-length Homoprotocatechuate 2,3-Dioxygenase from B. fuscum in a new spacegroup.'''
'''Structure of a full-length Homoprotocatechuate 2,3-Dioxygenase from B. fuscum in a new spacegroup.'''
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[[Category: Kovaleva, E G.]]
[[Category: Kovaleva, E G.]]
[[Category: Lipscomb, J D.]]
[[Category: Lipscomb, J D.]]
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[[Category: extradiol]]
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[[Category: Extradiol]]
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[[Category: fe(ii)]]
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[[Category: Homoprotocatechuate]]
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[[Category: homoprotocatechuate]]
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[[Category: Oxygenase]]
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[[Category: oxygenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:28:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:43:57 2008''
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Revision as of 04:28, 4 May 2008

Image:2ig9.gif

Template:STRUCTURE 2ig9

Structure of a full-length Homoprotocatechuate 2,3-Dioxygenase from B. fuscum in a new spacegroup.


Overview

We report the structures of three intermediates in the O2 activation and insertion reactions of an extradiol ring-cleaving dioxygenase. A crystal of Fe2+-containing homoprotocatechuate 2,3-dioxygenase was soaked in the slow substrate 4-nitrocatechol in a low O2 atmosphere. The x-ray crystal structure shows that three different intermediates reside in different subunits of a single homotetrameric enzyme molecule. One of these is the key substrate-alkylperoxo-Fe2+ intermediate, which has been predicted, but not structurally characterized, in an oxygenase. The intermediates define the major chemical steps of the dioxygenase mechanism and point to a general mechanistic strategy for the diverse 2-His-1-carboxylate enzyme family.

About this Structure

2IG9 is a Single protein structure of sequence from Brevibacterium fuscum. Full crystallographic information is available from OCA.

Reference

Crystal structures of Fe2+ dioxygenase superoxo, alkylperoxo, and bound product intermediates., Kovaleva EG, Lipscomb JD, Science. 2007 Apr 20;316(5823):453-7. PMID:17446402 Page seeded by OCA on Sun May 4 07:28:02 2008

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