2iif

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[[Image:2iif.gif|left|200px]]
[[Image:2iif.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2iif |SIZE=350|CAPTION= <scene name='initialview01'>2iif</scene>, resolution 2.720&Aring;
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The line below this paragraph, containing "STRUCTURE_2iif", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2iif| PDB=2iif | SCENE= }}
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|RELATEDENTRY=[[1ihf|1IHF]], [[2iie|2IIE]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iif OCA], [http://www.ebi.ac.uk/pdbsum/2iif PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iif RCSB]</span>
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}}
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'''single chain Integration Host Factor mutant protein (scIHF2-K45aE) in complex with DNA'''
'''single chain Integration Host Factor mutant protein (scIHF2-K45aE) in complex with DNA'''
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[[Category: Davey, C A.]]
[[Category: Davey, C A.]]
[[Category: Droege, P.]]
[[Category: Droege, P.]]
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[[Category: bending]]
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[[Category: Bending]]
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[[Category: divalent]]
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[[Category: Divalent]]
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[[Category: dna kinking]]
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[[Category: Dna kinking]]
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[[Category: intercalation]]
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[[Category: Intercalation]]
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[[Category: metal]]
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[[Category: Metal]]
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[[Category: u-turn]]
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[[Category: U-turn]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:32:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:44:53 2008''
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Revision as of 04:32, 4 May 2008

Image:2iif.gif

Template:STRUCTURE 2iif

single chain Integration Host Factor mutant protein (scIHF2-K45aE) in complex with DNA


Overview

Architectural proteins that reconfigure the paths of DNA segments are required for the establishment of functional interfaces in many genomic transactions. A single-chain derivative of the DNA architectural protein integration host factor was found to adopt two stable conformational states in complex with a specific DNA target. In the so-called open state, the degree of protein-induced DNA bending is reduced significantly compared with the closed state. The conformational switch between these states is controlled by divalent metal binding in two electronegative zones arising from the lysine-to-glutamate substitution in the protein body proximal to the phosphate backbone of one DNA arm. We show that this switch can be employed to control the efficiency of site-specific recombination catalyzed by lambda integrase. Introduction of acidic residues at the protein-DNA interface holds potential for the design of metal-mediated switches for the investigation of functional relationships.

About this Structure

2IIF is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A divalent metal-mediated switch controlling protein-induced DNA bending., Bao Q, Chen H, Liu Y, Yan J, Droge P, Davey CA, J Mol Biol. 2007 Mar 30;367(3):731-40. Epub 2006 Oct 3. PMID:17276457 Page seeded by OCA on Sun May 4 07:32:40 2008

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OCA

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