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2iu4
From Proteopedia
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[[Image:2iu4.gif|left|200px]] | [[Image:2iu4.gif|left|200px]] | ||
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'''DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ''' | '''DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ''' | ||
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[[Category: Erni, B.]] | [[Category: Erni, B.]] | ||
[[Category: Srinivas, A.]] | [[Category: Srinivas, A.]] | ||
| - | [[Category: | + | [[Category: Co-activator]] |
| - | [[Category: | + | [[Category: Dihydroxyacetone]] |
| - | [[Category: | + | [[Category: Kinase]] |
| - | [[Category: | + | [[Category: Lactococcus lacti]] |
| - | [[Category: | + | [[Category: Transferase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:52:59 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:52, 4 May 2008
DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ
Overview
Dihydroxyacetone (Dha) kinases are a novel family of kinases with signaling and metabolic functions. Here we report the x-ray structures of the transcriptional activator DhaS and the coactivator DhaQ and characterize their function. DhaQ is a paralog of the Dha binding Dha kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional activator. DhaQ and DhaS form a stable complex that in the presence of Dha activates transcription of the Lactococcus lactis dha operon. Dha covalently binds to DhaQ through a hemiaminal bond with a histidine and thereby induces a conformational change, which is propagated to the surface via a cantilever-like structure. DhaS binding protects an inverted repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs of the operator DNA hypersensitive to DNase I cleavage. The proximal half-site of the inverted repeat partially overlaps with the predicted -35 consensus sequence of the dha promoter.
About this Structure
2IU4 is a Single protein structure of sequence from Lactococcus lactis. Full crystallographic information is available from OCA.
Reference
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471 Page seeded by OCA on Sun May 4 07:52:59 2008
