2iv2

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[[Image:2iv2.gif|left|200px]]
[[Image:2iv2.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2iv2 |SIZE=350|CAPTION= <scene name='initialview01'>2iv2</scene>, resolution 2.27&Aring;
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The line below this paragraph, containing "STRUCTURE_2iv2", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:S+Binding+Site+For+Chain+X'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=2MD:GUANYLATE-O&#39;-PHOSPHORIC+ACID+MONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,5,6,7,8A,9,10,10A-OCTAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)+ESTER'>2MD</scene>, <scene name='pdbligand=CSE:SELENOCYSTEINE'>CSE</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=S:SULFUR+ATOM'>S</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2iv2| PDB=2iv2 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iv2 OCA], [http://www.ebi.ac.uk/pdbsum/2iv2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iv2 RCSB]</span>
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}}
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'''REINTERPRETATION OF REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''
'''REINTERPRETATION OF REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''
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==About this Structure==
==About this Structure==
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2IV2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IV2 OCA].
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2IV2 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IV2 OCA].
==Reference==
==Reference==
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[[Category: Romao, M J.]]
[[Category: Romao, M J.]]
[[Category: 4fe-4]]
[[Category: 4fe-4]]
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[[Category: anaerobic]]
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[[Category: Anaerobic]]
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[[Category: complete proteome]]
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[[Category: Complete proteome]]
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[[Category: dehydrogenase]]
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[[Category: Dehydrogenase]]
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[[Category: direct protein sequencing]]
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[[Category: Direct protein sequencing]]
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[[Category: fe4s4]]
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[[Category: Fe4s4]]
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[[Category: formate]]
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[[Category: Formate]]
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[[Category: iron]]
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[[Category: Iron]]
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[[Category: iron sulfur cluster]]
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[[Category: Iron sulfur cluster]]
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[[Category: iron-sulfur]]
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[[Category: Iron-sulfur]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: mgd]]
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[[Category: Mgd]]
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[[Category: molybdenum]]
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[[Category: Molybdenum]]
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[[Category: molybdopterin]]
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[[Category: Molybdopterin]]
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[[Category: molybdopterin guanine dinucleotide]]
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[[Category: Molybdopterin guanine dinucleotide]]
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[[Category: mpt]]
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[[Category: Mpt]]
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[[Category: nad]]
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[[Category: Nad]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: secy]]
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[[Category: Secy]]
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[[Category: selenium]]
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[[Category: Selenium]]
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[[Category: selenocysteine]]
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[[Category: Selenocysteine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:55:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:49:00 2008''
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Revision as of 04:55, 4 May 2008

Image:2iv2.gif

Template:STRUCTURE 2iv2

REINTERPRETATION OF REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI


Overview

Re-evaluation of the crystallographic data of the molybdenum-containing E. coli formate dehydrogenase H (Boyington et al. Science 275:1305-1308, 1997), reported in two redox states, reveals important structural differences for the formate-reduced form, with large implications for the reaction mechanism proposed in that work. We have re-refined the reduced structure with revised protocols and found substantial rearrangement in some parts of it. The original model is essentially correct but an important loop close to the molybdenum active site was mistraced, and, therefore, catalytic relevant residues were located in wrong positions. In particular selenocysteine-140, a ligand of molybdenum in the original work, and essential for catalysis, is no longer bound to the metal after reduction of the enzyme with formate. These results are incompatible with the originally proposed reaction mechanism. On the basis of our new interpretation, we have revised and proposed a new reaction mechanism, which reconciles the new X-ray model with previous biochemical and extended X-ray absorption fine structure data.

About this Structure

2IV2 is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Formate-reduced E. coli formate dehydrogenase H: The reinterpretation of the crystal structure suggests a new reaction mechanism., Raaijmakers HC, Romao MJ, J Biol Inorg Chem. 2006 Oct;11(7):849-54. Epub 2006 Jul 8. PMID:16830149 Page seeded by OCA on Sun May 4 07:55:27 2008

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