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2iw5

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[[Image:2iw5.gif|left|200px]]
[[Image:2iw5.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2iw5 |SIZE=350|CAPTION= <scene name='initialview01'>2iw5</scene>, resolution 2.57&Aring;
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The line below this paragraph, containing "STRUCTURE_2iw5", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2iw5| PDB=2iw5 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iw5 OCA], [http://www.ebi.ac.uk/pdbsum/2iw5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iw5 RCSB]</span>
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}}
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'''STRUCTURAL BASIS FOR COREST-DEPENDENT DEMETHYLATION OF NUCLEOSOMES BY THE HUMAN LSD1 HISTONE DEMETHYLASE'''
'''STRUCTURAL BASIS FOR COREST-DEPENDENT DEMETHYLATION OF NUCLEOSOMES BY THE HUMAN LSD1 HISTONE DEMETHYLASE'''
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[[Category: Yang, M.]]
[[Category: Yang, M.]]
[[Category: Yu, H.]]
[[Category: Yu, H.]]
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[[Category: alternative splicing]]
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[[Category: Alternative splicing]]
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[[Category: chromatin demethylation]]
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[[Category: Chromatin demethylation]]
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[[Category: chromatin regulator]]
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[[Category: Chromatin regulator]]
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[[Category: coiled coil]]
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[[Category: Coiled coil]]
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[[Category: corest]]
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[[Category: Corest]]
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[[Category: fad]]
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[[Category: Fad]]
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[[Category: histone demethylase]]
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[[Category: Histone demethylase]]
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[[Category: host-virus interaction]]
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[[Category: Host-virus interaction]]
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[[Category: lsd1]]
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[[Category: Lsd1]]
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[[Category: nuclear protein]]
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[[Category: Nuclear protein]]
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[[Category: nucleosome]]
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[[Category: Nucleosome]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: oxidoreductase/repressor complex]]
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[[Category: Oxidoreductase/repressor complex]]
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[[Category: phosphorylation]]
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[[Category: Phosphorylation]]
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[[Category: repressor]]
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[[Category: Repressor]]
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[[Category: transcription]]
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[[Category: Transcription]]
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[[Category: transcription regulation]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:58:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:49:27 2008''
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Revision as of 04:58, 4 May 2008

Image:2iw5.gif

Template:STRUCTURE 2iw5

STRUCTURAL BASIS FOR COREST-DEPENDENT DEMETHYLATION OF NUCLEOSOMES BY THE HUMAN LSD1 HISTONE DEMETHYLASE


Overview

Histone methylation regulates diverse chromatin-templated processes, including transcription. Many transcriptional corepressor complexes contain lysine-specific demethylase 1 (LSD1) and CoREST that collaborate to demethylate mono- and dimethylated H3-K4 of nucleosomes. Here, we report the crystal structure of the LSD1-CoREST complex. LSD1-CoREST forms an elongated structure with a long stalk connecting the catalytic domain of LSD1 and the CoREST SANT2 domain. LSD1 recognizes a large segment of the H3 tail through a deep, negatively charged pocket at the active site and possibly a shallow groove on its surface. CoREST SANT2 interacts with DNA. Disruption of the SANT2-DNA interaction diminishes CoREST-dependent demethylation of nucleosomes by LSD1. The shape and dimension of LSD1-CoREST suggest its bivalent binding to nucleosomes, allowing efficient H3-K4 demethylation. This spatially separated, multivalent nucleosome binding mode may apply to other chromatin-modifying enzymes that generally contain multiple nucleosome binding modules.

About this Structure

2IW5 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demethylase., Yang M, Gocke CB, Luo X, Borek D, Tomchick DR, Machius M, Otwinowski Z, Yu H, Mol Cell. 2006 Aug 4;23(3):377-87. PMID:16885027 Page seeded by OCA on Sun May 4 07:58:08 2008

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