2iyk
From Proteopedia
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'''CRYSTAL STRUCTURE OF THE UPF2-INTERACTING DOMAIN OF NONSENSE MEDIATED MRNA DECAY FACTOR UPF1''' | '''CRYSTAL STRUCTURE OF THE UPF2-INTERACTING DOMAIN OF NONSENSE MEDIATED MRNA DECAY FACTOR UPF1''' | ||
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[[Category: Kadlec, J.]] | [[Category: Kadlec, J.]] | ||
[[Category: Ravelli, R B.]] | [[Category: Ravelli, R B.]] | ||
| - | [[Category: | + | [[Category: Alternative splicing]] |
| - | [[Category: | + | [[Category: Atp-binding]] |
| - | [[Category: | + | [[Category: Helicase]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Metal-binding]] |
| - | [[Category: | + | [[Category: Nmd]] |
| - | [[Category: | + | [[Category: Nonsense mediated mrna decay]] |
| - | [[Category: | + | [[Category: Nonsense-mediated mrna decay]] |
| - | [[Category: | + | [[Category: Nucleotide-binding]] |
| - | [[Category: | + | [[Category: Phosphorylation]] |
| - | [[Category: | + | [[Category: Surveillance complex]] |
| - | [[Category: | + | [[Category: Upf1]] |
| - | [[Category: | + | [[Category: Zinc]] |
| - | [[Category: | + | [[Category: Zinc-finger]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:04:59 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:05, 4 May 2008
CRYSTAL STRUCTURE OF THE UPF2-INTERACTING DOMAIN OF NONSENSE MEDIATED MRNA DECAY FACTOR UPF1
Overview
UPF1 is an essential eukaryotic RNA helicase that plays a key role in various mRNA degradation pathways, notably nonsense-mediated mRNA decay (NMD). In combination with UPF2 and UPF3, it forms part of the surveillance complex that detects mRNAs containing premature stop codons and triggers their degradation in all organisms studied from yeast to human. We describe the 3 A resolution crystal structure of the highly conserved cysteine-histidine-rich domain of human UPF1 and show that it is a unique combination of three zinc-binding motifs arranged into two tandem modules related to the RING-box and U-box domains of ubiquitin ligases. This UPF1 domain interacts with UPF2, and we identified by mutational analysis residues in two distinct conserved surface regions of UPF1 that mediate this interaction. UPF1 residues we identify as important for the interaction with UPF2 are not conserved in UPF1 homologs from certain unicellular parasites that also appear to lack UPF2 in their genomes.
About this Structure
2IYK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the UPF2-interacting domain of nonsense-mediated mRNA decay factor UPF1., Kadlec J, Guilligay D, Ravelli RB, Cusack S, RNA. 2006 Oct;12(10):1817-24. Epub 2006 Aug 24. PMID:16931876 Page seeded by OCA on Sun May 4 08:04:59 2008
Categories: Homo sapiens | Single protein | Cusack, S. | Guilligay, D. | Kadlec, J. | Ravelli, R B. | Alternative splicing | Atp-binding | Helicase | Hydrolase | Metal-binding | Nmd | Nonsense mediated mrna decay | Nonsense-mediated mrna decay | Nucleotide-binding | Phosphorylation | Surveillance complex | Upf1 | Zinc | Zinc-finger
