2j1p
From Proteopedia
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| - | + | {{STRUCTURE_2j1p| PDB=2j1p | SCENE= }} | |
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'''GERANYLGERANYL DIPHOSPHATE SYNTHASE FROM SINAPIS ALBA IN COMPLEX WITH GGPP''' | '''GERANYLGERANYL DIPHOSPHATE SYNTHASE FROM SINAPIS ALBA IN COMPLEX WITH GGPP''' | ||
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[[Category: Schulz, G E.]] | [[Category: Schulz, G E.]] | ||
[[Category: Welsch, R.]] | [[Category: Welsch, R.]] | ||
| - | [[Category: | + | [[Category: Carotenoid biosynthesis]] |
| - | [[Category: | + | [[Category: Chloroplast]] |
| - | [[Category: | + | [[Category: Geranylgeranyl diphosphate]] |
| - | [[Category: | + | [[Category: Isoprene biosynthesis]] |
| - | [[Category: | + | [[Category: Isoprenoid diphosphate synthase]] |
| - | [[Category: | + | [[Category: Isoprenyl transtransferase]] |
| - | [[Category: | + | [[Category: Multifunctional enzyme]] |
| - | [[Category: | + | [[Category: Plastid]] |
| - | [[Category: | + | [[Category: Transferase]] |
| - | [[Category: | + | [[Category: Transit peptide]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:13:24 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:13, 4 May 2008
GERANYLGERANYL DIPHOSPHATE SYNTHASE FROM SINAPIS ALBA IN COMPLEX WITH GGPP
Overview
The crystal structure of the geranylgeranyl diphosphate synthase from Sinapis alba (mustard) has been solved in two crystal forms at 1.8 and 2.0 A resolutions. In one of these forms, the dimeric enzyme binds one molecule of the final product geranylgeranyl diphosphate in one subunit. The chainfold of the enzyme corresponds to that of other members of the farnesyl diphosphate synthase family. Whereas the binding modes of the two substrates dimethylallyl diphosphate and isopentenyl diphosphate at the allyl and isopentenyl sites, respectively, have been established with other members of the family, the complex structure presented reveals for the first time the binding mode of a reaction product at the isopentenyl site. The binding geometry of substrates and product in conjunction with the protein environment and the established chemistry of the reaction provide a clear picture of the reaction steps and atom displacements. Moreover, a comparison with a ligated homologous structure outlined an appreciable induced fit: helix alpha8 and its environment undergo a large conformational change when either the substrate dimethylallyl diphosphate or an analogue is bound to the allyl site; only a minor conformational change occurs when the other substrate isopentenyl diphosphate or the product is bound to the isopentenyl site.
About this Structure
2J1P is a Single protein structure of sequence from Sinapis alba. Full crystallographic information is available from OCA.
Reference
Structure and reaction geometry of geranylgeranyl diphosphate synthase from Sinapis alba., Kloer DP, Welsch R, Beyer P, Schulz GE, Biochemistry. 2006 Dec 26;45(51):15197-204. Epub 2006 Dec 1. PMID:17176041 Page seeded by OCA on Sun May 4 08:13:24 2008
Categories: Sinapis alba | Single protein | Beyer, P. | Kloer, D P. | Schulz, G E. | Welsch, R. | Carotenoid biosynthesis | Chloroplast | Geranylgeranyl diphosphate | Isoprene biosynthesis | Isoprenoid diphosphate synthase | Isoprenyl transtransferase | Multifunctional enzyme | Plastid | Transferase | Transit peptide
